Detailed information of TA system TA056478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 25315..25922
• Replicon: plasmid ISMMS_VRE_p1
• Accession: NZ_CP012432
• Organism: Enterococcus faecium strain ISMMS_VRE_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: AL014_RS15900
• Protein ID: WP_002321032.1
• Coordinates: 25572..25922 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: AL014_RS15895
• Protein ID: WP_002287514.1
• Coordinates: 25315..25578 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL014_RS15855	20665..21138	+	474	WP_002321302.1	single-stranded DNA-binding protein	-
AL014_RS15860	21504..21764	+	261	WP_002321303.1	hypothetical protein	-
AL014_RS15865	22209..22415	+	207	WP_002296239.1	hypothetical protein	-
AL014_RS15870	22415..22666	+	252	WP_002305766.1	hypothetical protein	-
AL014_RS15875	22682..23098	+	417	WP_002305764.1	hypothetical protein	-
AL014_RS15880	23080..23352	+	273	WP_002348912.1	hypothetical protein	-
AL014_RS15885	23519..23920	+	402	WP_002305759.1	IS200/IS605 family transposase	-
AL014_RS15890	23932..25077	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
AL014_RS15895	25315..25578	+	264	WP_002287514.1	hypothetical protein	Antitoxin
AL014_RS15900	25572..25922	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL014_RS15905	25946..26560	-	615	WP_002318230.1	recombinase family protein	-
AL014_RS15910	27010..28335	+	1326	WP_002348911.1	Y-family DNA polymerase	-
AL014_RS15915	28328..28678	+	351	WP_002303114.1	hypothetical protein	-
AL014_RS15920	28675..28854	+	180	WP_002322386.1	hypothetical protein	-
AL014_RS15925	28866..29159	+	294	WP_002322251.1	hypothetical protein	-
AL014_RS15930	29385..30203	+	819	WP_002305212.1	ParA family protein	-
AL014_RS15935	30222..30629	+	408	WP_002305774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57543	57543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T56478 WP_002321032.1 NZ_CP012432:25572-25922 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T56478 NZ_CP012432:25572-25922 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT56478 WP_002287514.1 NZ_CP012432:25315-25578 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT56478 NZ_CP012432:25315-25578 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0