Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2242264..2242793 Replicon chromosome
Accession NZ_CP012430
Organism Enterococcus faecium strain ISMMS_VRE_1

Toxin (Protein)


Gene name relE Uniprot ID Q3XX67
Locus tag AL014_RS11225 Protein ID WP_002296560.1
Coordinates 2242264..2242530 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A829F3T0
Locus tag AL014_RS11230 Protein ID WP_002296559.1
Coordinates 2242530..2242793 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL014_RS11195 2237664..2238068 - 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -
AL014_RS11200 2238226..2238807 + 582 Protein_2187 CBS domain-containing protein -
AL014_RS11205 2238811..2239452 + 642 WP_002293405.1 ABC transporter permease -
AL014_RS11210 2239449..2240366 + 918 WP_002296564.1 osmoprotectant ABC transporter substrate-binding protein -
AL014_RS11215 2240363..2241034 + 672 WP_002302663.1 ABC transporter permease -
AL014_RS11220 2241575..2242063 - 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
AL014_RS11225 2242264..2242530 - 267 WP_002296560.1 Txe/YoeB family addiction module toxin Toxin
AL014_RS11230 2242530..2242793 - 264 WP_002296559.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL014_RS11235 2243269..2243454 + 186 WP_002296558.1 transcriptional regulator -
AL014_RS11240 2243596..2245419 - 1824 WP_002294202.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10660.13 Da        Isoelectric Point: 9.9931

>T56477 WP_002296560.1 NZ_CP012430:c2242530-2242264 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T56477 NZ_CP012430:c2242530-2242264 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10046.05 Da        Isoelectric Point: 4.1207

>AT56477 WP_002296559.1 NZ_CP012430:c2242793-2242530 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNID
DIDWEAL

Download         Length: 264 bp

>AT56477 NZ_CP012430:c2242793-2242530 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FCW8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3T0

References