Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1919009..1919580 Replicon chromosome
Accession NZ_CP012430
Organism Enterococcus faecium strain ISMMS_VRE_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag AL014_RS09700 Protein ID WP_002286801.1
Coordinates 1919009..1919350 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag AL014_RS09705 Protein ID WP_002323011.1
Coordinates 1919350..1919580 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL014_RS09675 1914219..1915553 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
AL014_RS09680 1915761..1916591 + 831 WP_002286789.1 manganese catalase family protein -
AL014_RS16835 1916835..1917117 - 283 Protein_1884 transposase -
AL014_RS09690 1917275..1917469 - 195 WP_002297028.1 hypothetical protein -
AL014_RS09695 1917974..1918159 - 186 WP_002304207.1 hypothetical protein -
AL014_RS09700 1919009..1919350 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AL014_RS09705 1919350..1919580 - 231 WP_002323011.1 hypothetical protein Antitoxin
AL014_RS09710 1919867..1920205 + 339 WP_002286804.1 hypothetical protein -
AL014_RS09715 1920410..1920649 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
AL014_RS09720 1920625..1920972 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
AL014_RS09725 1921704..1922285 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
AL014_RS09730 1922468..1923094 - 627 WP_002286816.1 cysteine hydrolase -
AL014_RS09735 1923116..1924447 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T56476 WP_002286801.1 NZ_CP012430:c1919350-1919009 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T56476 NZ_CP012430:c1919350-1919009 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT56476 WP_002323011.1 NZ_CP012430:c1919580-1919350 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT56476 NZ_CP012430:c1919580-1919350 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References