Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4293668..4294404 Replicon chromosome
Accession NZ_CP012426
Organism Klebsiella pneumoniae strain KP5

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag ACG94_RS21825 Protein ID WP_032433360.1
Coordinates 4293922..4294404 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag ACG94_RS21820 Protein ID WP_003026799.1
Coordinates 4293668..4293934 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACG94_RS21795 4289314..4290453 + 1140 WP_032433368.1 mannitol dehydrogenase -
ACG94_RS21800 4290482..4291144 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
ACG94_RS21805 4291128..4292132 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
ACG94_RS21810 4292150..4292782 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
ACG94_RS21815 4292792..4293355 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
ACG94_RS21820 4293668..4293934 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
ACG94_RS21825 4293922..4294404 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
ACG94_RS21830 4294562..4296094 - 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
ACG94_RS21835 4296206..4297609 + 1404 WP_039815096.1 ISNCY-like element ISKpn21 family transposase -
ACG94_RS21840 4297638..4298270 - 633 WP_001567369.1 hypothetical protein -
ACG94_RS21845 4298650..4299249 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4263890..4309232 45342


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T56466 WP_032433360.1 NZ_CP012426:4293922-4294404 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T56466 NZ_CP012426:4293922-4294404 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT56466 WP_003026799.1 NZ_CP012426:4293668-4293934 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT56466 NZ_CP012426:4293668-4293934 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References