Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4276976..4277619 Replicon chromosome
Accession NZ_CP012426
Organism Klebsiella pneumoniae strain KP5

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag ACG94_RS21735 Protein ID WP_032433387.1
Coordinates 4277203..4277619 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag ACG94_RS21730 Protein ID WP_001261276.1
Coordinates 4276976..4277206 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACG94_RS21710 4271998..4273085 + 1088 Protein_4036 transcriptional regulator -
ACG94_RS21715 4273088..4275328 + 2241 WP_045326788.1 NTPase -
ACG94_RS21725 4275856..4276671 - 816 WP_032433388.1 hypothetical protein -
ACG94_RS21730 4276976..4277206 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ACG94_RS21735 4277203..4277619 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
ACG94_RS21740 4277775..4278755 + 981 WP_032433385.1 hypothetical protein -
ACG94_RS21745 4278950..4280521 - 1572 WP_032433383.1 ATP-binding protein -
ACG94_RS21750 4280840..4281088 + 249 WP_032433382.1 hypothetical protein -
ACG94_RS21755 4281147..4281665 + 519 WP_045326794.1 hypothetical protein -
ACG94_RS21760 4281696..4282187 + 492 WP_032433378.1 hypothetical protein -
ACG94_RS29440 4282247..4282451 + 205 Protein_4046 hemolysin expression modulator Hha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4263890..4309232 45342


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T56465 WP_032433387.1 NZ_CP012426:4277203-4277619 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T56465 NZ_CP012426:4277203-4277619 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT56465 WP_001261276.1 NZ_CP012426:4276976-4277206 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT56465 NZ_CP012426:4276976-4277206 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References