Detailed information of TA system TA056452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 14353..14959
• Replicon: plasmid pPSA1-4
• Accession: NZ_CP012417
• Organism: Piscirickettsia salmonis strain PM15972A1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: KW89_RS16440
• Protein ID: WP_027242955.1
• Coordinates: 14699..14959 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KW89_RS16435
• Protein ID: WP_054300685.1
• Coordinates: 14353..14706 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW89_RS16410	9812..10597	-	786	WP_032126922.1	queuosine precursor transporter	-
KW89_RS16415	10581..11012	-	432	WP_016211069.1	hypothetical protein	-
KW89_RS18165	11037..11180	-	144	WP_155047030.1	hypothetical protein	-
KW89_RS16420	11395..12636	+	1242	WP_054300683.1	MFS transporter	-
KW89_RS16425	12640..13452	+	813	WP_016211068.1	LuxR C-terminal-related transcriptional regulator	-
KW89_RS16430	13449..14249	-	801	WP_054300684.1	methyltransferase domain-containing protein	-
KW89_RS16435	14353..14706	-	354	WP_054300685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KW89_RS16440	14699..14959	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
KW89_RS16445	15221..15688	-	468	WP_036781099.1	hypothetical protein	-
KW89_RS16450	15702..15935	-	234	WP_036781101.1	hypothetical protein	-
KW89_RS16455	15978..16232	-	255	WP_054300687.1	DUF4160 domain-containing protein	-
KW89_RS16460	16596..17963	-	1368	WP_054300688.1	hypothetical protein	-
KW89_RS16465	17980..18972	-	993	WP_054300689.1	ParA family protein	-
KW89_RS16470	19611..19835	+	225	Protein_32	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31951	31951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T56452 WP_027242955.1 NZ_CP012417:c14959-14699 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T56452 NZ_CP012417:c14959-14699 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13462.36 Da
• Isoelectric Point: 5.0200

>AT56452 WP_054300685.1 NZ_CP012417:c14706-14353 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGGDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT56452 NZ_CP012417:c14706-14353 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTGGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTAAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87