Detailed information of TA system TA056451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 48886..49456
• Replicon: plasmid pPSA1-3
• Accession: NZ_CP012416
• Organism: Piscirickettsia salmonis strain PM15972A1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KW89_RS16140
• Protein ID: WP_054300674.1
• Coordinates: 48886..49212 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KW89_RS16145
• Protein ID: WP_054300649.1
• Coordinates: 49214..49456 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW89_RS18375	44816..44992	-	177	WP_172673426.1	transposase	-
KW89_RS16120	45067..45222	-	156	Protein_50	DNA-binding protein	-
KW89_RS16125	45396..45695	+	300	WP_075273685.1	HU family DNA-binding protein	-
KW89_RS16130	45808..47508	+	1701	WP_054300647.1	DUF4338 domain-containing protein	-
KW89_RS16135	47534..48769	+	1236	WP_054300648.1	hypothetical protein	-
KW89_RS16140	48886..49212	-	327	WP_054300674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KW89_RS16145	49214..49456	-	243	WP_054300649.1	hypothetical protein	Antitoxin
KW89_RS16150	49870..50190	-	321	WP_054300650.1	hypothetical protein	-
KW89_RS16155	50211..50645	-	435	WP_054300651.1	hypothetical protein	-
KW89_RS16160	50652..51059	-	408	WP_054300652.1	hypothetical protein	-
KW89_RS16165	51158..51466	-	309	WP_081007087.1	helix-turn-helix transcriptional regulator	-
KW89_RS16170	51516..51776	-	261	WP_054300654.1	hypothetical protein	-
KW89_RS16175	51838..52395	-	558	WP_054300675.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82130	82130
	-	flank	IS/Tn	-	-	51838..52416	578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12091.96 Da
• Isoelectric Point: 9.9714

>T56451 WP_054300674.1 NZ_CP012416:c49212-48886 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMFRG

Nucleotide

• Length: 327 bp

>T56451 NZ_CP012416:c49212-48886 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTAACTTCCAAGCCTTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGCGCAGTGCTAGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGACTGGAATCATGCTGATAAGCTCGATAAAAAATCACTTGAAAGAGTTCAAGGTTTAATTGACGTGATGTTTAG
AGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.14 Da
• Isoelectric Point: 4.6318

>AT56451 WP_054300649.1 NZ_CP012416:c49456-49214 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTKTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT56451 NZ_CP012416:c49456-49214 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGCTTGGGTTTATTATTGCCATTGAGCATTGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACGCATACGAAGACCCTAGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA