Detailed information of TA system TA056450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 24335..24941
• Replicon: plasmid pPSA1-1
• Accession: NZ_CP012414
• Organism: Piscirickettsia salmonis strain PM15972A1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: KW89_RS15585
• Protein ID: WP_027242955.1
• Coordinates: 24681..24941 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KW89_RS15580
• Protein ID: WP_036780061.1
• Coordinates: 24335..24688 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW89_RS15560	20764..21789	+	1026	WP_052104629.1	IS91 family transposase	-
KW89_RS15565	21964..22422	-	459	WP_054300586.1	hypothetical protein	-
KW89_RS15575	22847..23872	+	1026	WP_052104629.1	IS91 family transposase	-
KW89_RS15580	24335..24688	-	354	WP_036780061.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KW89_RS15585	24681..24941	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
KW89_RS17475	25190..26164	+	975	WP_054300271.1	IS30 family transposase	-
KW89_RS15595	26341..27573	-	1233	WP_032126623.1	MFS transporter	-
KW89_RS15600	27686..28711	-	1026	WP_032126388.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39335	39335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T56450 WP_027242955.1 NZ_CP012414:c24941-24681 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T56450 NZ_CP012414:c24941-24681 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.44 Da
• Isoelectric Point: 5.0200

>AT56450 WP_036780061.1 NZ_CP012414:c24688-24335 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQIVKR

Nucleotide

• Length: 354 bp

>AT56450 NZ_CP012414:c24688-24335 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTGAAGCATGATCAAATCGTTAAACGGTAA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87