Detailed information of TA system TA056449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5362..6005
• Replicon: plasmid pPSA1-1
• Accession: NZ_CP012414
• Organism: Piscirickettsia salmonis strain PM15972A1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: KW89_RS15465
• Protein ID: WP_016210974.1
• Coordinates: 5362..5769 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: KW89_RS15470
• Protein ID: WP_016210972.1
• Coordinates: 5778..6005 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW89_RS15435	1697..2176	-	480	WP_016212234.1	P27 family phage terminase small subunit	-
KW89_RS15440	2264..2647	-	384	WP_032126134.1	HNH endonuclease	-
KW89_RS15445	2957..3322	-	366	WP_016212235.1	hypothetical protein	-
KW89_RS18140	3369..3548	+	180	WP_155046773.1	phosphatase	-
KW89_RS18365	3815..4093	-	279	WP_032126476.1	hypothetical protein	-
KW89_RS15455	4093..4386	-	294	WP_016210977.1	zinc ribbon domain-containing protein	-
KW89_RS15460	4565..5245	-	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
KW89_RS15465	5362..5769	-	408	WP_016210974.1	DUF4411 family protein	Toxin
KW89_RS15470	5778..6005	-	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KW89_RS17455	6142..7116	-	975	WP_054300271.1	IS30 family transposase	-
KW89_RS15480	7342..8433	-	1092	WP_054300578.1	hypothetical protein	-
KW89_RS15485	8964..9428	-	465	WP_016212274.1	type II toxin-antitoxin system HicB family antitoxin	-
KW89_RS15490	9439..9633	-	195	WP_032126154.1	type II toxin-antitoxin system HicA family toxin	-
KW89_RS15495	9849..10439	+	591	WP_032126152.1	site-specific integrase	-
KW89_RS15500	10503..10844	+	342	WP_081007075.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39335	39335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T56449 WP_016210974.1 NZ_CP012414:c5769-5362 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T56449 NZ_CP012414:c5769-5362 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT56449 WP_016210972.1 NZ_CP012414:c6005-5778 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT56449 NZ_CP012414:c6005-5778 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5