Detailed information of TA system TA056441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2256308..2256837
• Replicon: chromosome
• Accession: NZ_CP012409
• Organism: Staphylococcus aureus subsp. aureus Tager 104

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Tgr_RS11185
• Protein ID: WP_000621175.1
• Coordinates: 2256475..2256837 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: Tgr_RS11180
• Protein ID: WP_000948331.1
• Coordinates: 2256308..2256478 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tgr_RS11150	2251344..2251904	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
Tgr_RS11155	2252113..2252592	+	480	WP_001287083.1	hypothetical protein	-
Tgr_RS11160	2252585..2254168	+	1584	WP_001294642.1	PH domain-containing protein	-
Tgr_RS11165	2254155..2254646	+	492	WP_001205916.1	PH domain-containing protein	-
Tgr_RS11170	2254650..2255009	+	360	WP_000581197.1	holo-ACP synthase	-
Tgr_RS11175	2255075..2256223	+	1149	WP_001281148.1	alanine racemase	-
Tgr_RS11180	2256308..2256478	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
Tgr_RS11185	2256475..2256837	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Tgr_RS11195	2257187..2258188	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
Tgr_RS11200	2258307..2258633	+	327	WP_001052491.1	anti-sigma factor antagonist	-
Tgr_RS11205	2258635..2259114	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
Tgr_RS11210	2259089..2259859	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T56441 WP_000621175.1 NZ_CP012409:2256475-2256837 [Staphylococcus aureus subsp. aureus Tager 104]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T56441 NZ_CP012409:2256475-2256837 [Staphylococcus aureus subsp. aureus Tager 104]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT56441 WP_000948331.1 NZ_CP012409:2256308-2256478 [Staphylococcus aureus subsp. aureus Tager 104]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT56441 NZ_CP012409:2256308-2256478 [Staphylococcus aureus subsp. aureus Tager 104]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1