Detailed information of TA system TA056440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2236579..2237093
• Replicon: chromosome
• Accession: NZ_CP012409
• Organism: Staphylococcus aureus subsp. aureus Tager 104

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: Tgr_RS11095
• Protein ID: WP_000581792.1
• Coordinates: 2236884..2237093 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: Tgr_RS11090
• Protein ID: WP_000138934.1
• Coordinates: 2236579..2236872 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tgr_RS11070	2231742..2232383	+	642	WP_029753888.1	thiamine phosphate synthase	-
Tgr_RS11075	2232469..2233341	+	873	WP_000725802.1	membrane protein insertase YidC	-
Tgr_RS11080	2234255..2234902	-	648	WP_001187619.1	HD domain-containing protein	-
Tgr_RS11085	2234930..2236413	-	1484	Protein_2122	cardiolipin synthase	-
Tgr_RS11090	2236579..2236872	+	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
Tgr_RS11095	2236884..2237093	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
Tgr_RS11100	2237221..2237358	+	138	WP_000828354.1	hypothetical protein	-
Tgr_RS11105	2237622..2238824	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
Tgr_RS11110	2239142..2240212	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
Tgr_RS11115	2240227..2241585	+	1359	WP_000611467.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T56440 WP_000581792.1 NZ_CP012409:2236884-2237093 [Staphylococcus aureus subsp. aureus Tager 104]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T56440 NZ_CP012409:2236884-2237093 [Staphylococcus aureus subsp. aureus Tager 104]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT56440 WP_000138934.1 NZ_CP012409:2236579-2236872 [Staphylococcus aureus subsp. aureus Tager 104]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT56440 NZ_CP012409:2236579-2236872 [Staphylococcus aureus subsp. aureus Tager 104]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9