Detailed information of TA system TA056408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3813830..3814630
• Replicon: chromosome
• Accession: NZ_CP012380
• Organism: Escherichia coli strain WAT

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: AKO64_RS19980
• Protein ID: WP_000342449.1
• Coordinates: 3813830..3814357 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: AKO64_RS19985
• Protein ID: WP_001277108.1
• Coordinates: 3814364..3814630 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKO64_RS19950	3808905..3809672	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AKO64_RS19955	3809669..3810946	-	1278	WP_000803771.1	branched chain amino acid ABC transporter permease LivM	-
AKO64_RS19960	3810943..3811869	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AKO64_RS19965	3811917..3813026	-	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AKO64_RS19975	3813450..3813833	+	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AKO64_RS19980	3813830..3814357	-	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
AKO64_RS19985	3814364..3814630	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
AKO64_RS19990	3814780..3815883	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AKO64_RS19995	3816154..3817008	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
AKO64_RS20000	3817253..3818311	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
AKO64_RS20005	3818304..3818972	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T56408 WP_000342449.1 NZ_CP012380:c3814357-3813830 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T56408 NZ_CP012380:c3814357-3813830 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT56408 WP_001277108.1 NZ_CP012380:c3814630-3814364 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT56408 NZ_CP012380:c3814630-3814364 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24