Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3239659..3240313 Replicon chromosome
Accession NZ_CP012380
Organism Escherichia coli strain WAT

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AKO64_RS16860 Protein ID WP_000244772.1
Coordinates 3239659..3240066 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AKO64_RS16865 Protein ID WP_000354046.1
Coordinates 3240047..3240313 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO64_RS16840 3235616..3237349 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
AKO64_RS16845 3237355..3238065 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AKO64_RS16850 3238090..3238986 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AKO64_RS16855 3239098..3239619 + 522 WP_001055874.1 flavodoxin FldB -
AKO64_RS16860 3239659..3240066 - 408 WP_000244772.1 protein YgfX Toxin
AKO64_RS16865 3240047..3240313 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AKO64_RS16870 3240556..3241536 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AKO64_RS16875 3241613..3242272 - 660 WP_000250274.1 hemolysin III family protein -
AKO64_RS16880 3242436..3242747 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
AKO64_RS16885 3242792..3244225 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
AKO64_RS16890 3244282..3245025 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T56406 WP_000244772.1 NZ_CP012380:c3240066-3239659 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T56406 NZ_CP012380:c3240066-3239659 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT56406 WP_000354046.1 NZ_CP012380:c3240313-3240047 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT56406 NZ_CP012380:c3240313-3240047 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References