Detailed information of TA system TA056404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2696346..2696971
• Replicon: chromosome
• Accession: NZ_CP012380
• Organism: Escherichia coli strain WAT

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AKO64_RS14005
• Protein ID: WP_000911330.1
• Coordinates: 2696346..2696744 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AKO64_RS14010
• Protein ID: WP_000450524.1
• Coordinates: 2696744..2696971 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKO64_RS13985	2692224..2692424	+	201	WP_000383836.1	YpfN family protein	-
AKO64_RS13990	2692534..2693232	-	699	WP_000679823.1	esterase	-
AKO64_RS13995	2693306..2695321	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
AKO64_RS14000	2695336..2696199	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
AKO64_RS14005	2696346..2696744	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AKO64_RS14010	2696744..2696971	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AKO64_RS14015	2697125..2697838	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AKO64_RS14020	2698051..2699085	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AKO64_RS14025	2699102..2699980	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AKO64_RS14030	2700126..2700698	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AKO64_RS14035	2700698..2701168	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(2')-IIa	-	2634352..2763080	128728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T56404 WP_000911330.1 NZ_CP012380:c2696744-2696346 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T56404 NZ_CP012380:c2696744-2696346 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT56404 WP_000450524.1 NZ_CP012380:c2696971-2696744 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT56404 NZ_CP012380:c2696971-2696744 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33