56392

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1546031..1546669 Replicon chromosome
Accession NZ_CP012380
Organism Escherichia coli strain WAT

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AKO64_RS07975 Protein ID WP_000813794.1
Coordinates 1546031..1546207 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AKO64_RS07980 Protein ID WP_001270286.1
Coordinates 1546253..1546669 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO64_RS07955 1541650..1542864 - 1215 WP_001349372.1 BenE family transporter YdcO -
AKO64_RS07960 1542917..1543453 + 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
AKO64_RS07965 1543526..1545487 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AKO64_RS07970 1545579..1545809 - 231 WP_000494244.1 YncJ family protein -
AKO64_RS07975 1546031..1546207 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AKO64_RS07980 1546253..1546669 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AKO64_RS07985 1546748..1548154 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
AKO64_RS07990 1548399..1549544 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
AKO64_RS07995 1549562..1550575 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AKO64_RS08000 1550576..1551517 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1540345..1541610 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T56392 WP_000813794.1 NZ_CP012380:1546031-1546207 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T56392 NZ_CP012380:1546031-1546207 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT56392 WP_001270286.1 NZ_CP012380:1546253-1546669 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT56392 NZ_CP012380:1546253-1546669 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References