Detailed information of TA system TA056379

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3201857..3202692
• Replicon: chromosome
• Accession: NZ_CP012379
• Organism: Escherichia coli strain PAR

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AKO63_RS16400
• Protein ID: WP_073533316.1
• Coordinates: 3202315..3202692 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: AKO63_RS16395
• Protein ID: WP_001278232.1
• Coordinates: 3201857..3202225 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKO63_RS16355	3198693..3199148	+	456	WP_073533319.1	hypothetical protein	-
AKO63_RS16360	3199227..3199460	+	234	WP_073533318.1	DUF905 family protein	-
AKO63_RS16370	3199560..3200378	+	819	WP_001234620.1	DUF945 domain-containing protein	-
AKO63_RS16375	3200433..3200918	+	486	WP_029701480.1	antirestriction protein	-
AKO63_RS16380	3200934..3201410	+	477	WP_149506776.1	RadC family protein	-
AKO63_RS16385	3201473..3201694	+	222	WP_000692312.1	DUF987 domain-containing protein	-
AKO63_RS16395	3201857..3202225	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
AKO63_RS16400	3202315..3202692	+	378	WP_073533316.1	TA system toxin CbtA family protein	Toxin
AKO63_RS16405	3202689..3203177	+	489	WP_073533309.1	hypothetical protein	-
AKO63_RS16410	3203189..3203386	+	198	Protein_3019	hypothetical protein	-
AKO63_RS16415	3203471..3204313	+	843	WP_073533308.1	DUF4942 domain-containing protein	-
AKO63_RS16420	3204717..3204938	+	222	Protein_3021	IS110 family transposase	-
AKO63_RS16425	3205697..3206680	+	984	WP_001338017.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14048.02 Da
• Isoelectric Point: 7.8045

>T56379 WP_073533316.1 NZ_CP012379:3202315-3202692 [Escherichia coli]
MKTLPDTHVQEASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T56379 NZ_CP012379:3202315-3202692 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACAGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT56379 WP_001278232.1 NZ_CP012379:3201857-3202225 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT56379 NZ_CP012379:3201857-3202225 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: E3PJ72