Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 31573..32132 Replicon plasmid pGE3
Accession NZ_CP012377
Organism Escherichia coli strain GE3 isolate human faeces

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag AKN41_RS24105 Protein ID WP_001620845.1
Coordinates 31573..31848 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag AKN41_RS24110 Protein ID WP_024188746.1
Coordinates 31848..32132 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKN41_RS24080 28444..29478 - 1035 WP_001620850.1 3-deoxy-7-phosphoheptulonate synthase -
AKN41_RS24090 30038..30346 - 309 WP_042110166.1 hypothetical protein -
AKN41_RS24095 30501..30737 - 237 WP_032257624.1 colicin V immunity protein -
AKN41_RS24100 31187..31468 - 282 WP_001105066.1 hypothetical protein -
AKN41_RS24105 31573..31848 - 276 WP_001620845.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AKN41_RS24110 31848..32132 - 285 WP_024188746.1 ribbon-helix-helix domain-containing protein Antitoxin
AKN41_RS24125 33047..33904 - 858 WP_001774315.1 incFII family plasmid replication initiator RepA -
AKN41_RS24130 33897..33971 - 75 WP_001367777.1 RepA leader peptide Tap -
AKN41_RS24140 34205..34462 - 258 WP_032082917.1 replication regulatory protein RepA -
AKN41_RS24145 34702..35292 - 591 WP_042109322.1 DUF2726 domain-containing protein -
AKN41_RS24150 35330..35539 - 210 WP_001333231.1 hemolysin expression modulator Hha -
AKN41_RS24155 35585..36046 - 462 WP_001233868.1 thermonuclease family protein -
AKN41_RS24160 36295..36507 - 213 WP_032197545.1 ANR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iroB / iroC / iroD / iroE / iroN / papC / papH 1..125121 125121


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10763.47 Da        Isoelectric Point: 9.9324

>T56355 WP_001620845.1 NZ_CP012377:c31848-31573 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREIRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T56355 NZ_CP012377:c31848-31573 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAAGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAGCCCAGGGAGATCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAATAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10446.88 Da        Isoelectric Point: 5.6558

>AT56355 WP_024188746.1 NZ_CP012377:c32132-31848 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAAKLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT56355 NZ_CP012377:c32132-31848 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAAACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References