Detailed information of TA system TA056354

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4804555..4805387
• Replicon: chromosome
• Accession: NZ_CP012376
• Organism: Escherichia coli strain GE3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: AKN41_RS23460
• Protein ID: WP_000854753.1
• Coordinates: 4805013..4805387 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: AKN41_RS23455
• Protein ID: WP_001315620.1
• Coordinates: 4804555..4804923 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKN41_RS23420	4801391..4801846	+	456	WP_000581504.1	hypothetical protein	-
AKN41_RS23425	4801925..4802158	+	234	WP_001119729.1	DUF905 family protein	-
AKN41_RS23435	4802258..4803076	+	819	WP_001234620.1	DUF945 domain-containing protein	-
AKN41_RS23440	4803131..4803616	+	486	WP_000849588.1	antirestriction protein	-
AKN41_RS23445	4803632..4804108	+	477	WP_001560709.1	RadC family protein	-
AKN41_RS23450	4804171..4804392	+	222	WP_000692345.1	DUF987 domain-containing protein	-
AKN41_RS23455	4804555..4804923	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AKN41_RS23460	4805013..4805387	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
AKN41_RS23465	4805384..4805872	+	489	WP_000777547.1	hypothetical protein	-
AKN41_RS23470	4805884..4806081	+	198	WP_000839281.1	DUF957 domain-containing protein	-
AKN41_RS24815	4806166..4806318	+	153	WP_001280445.1	hypothetical protein	-
AKN41_RS23495	4807363..4808013	+	651	WP_001037966.1	HNH endonuclease	-
AKN41_RS23500	4808021..4809001	-	981	WP_001295601.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
AKN41_RS23505	4809066..4810172	-	1107	WP_001315214.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T56354 WP_000854753.1 NZ_CP012376:4805013-4805387 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T56354 NZ_CP012376:4805013-4805387 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT56354 WP_001315620.1 NZ_CP012376:4804555-4804923 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT56354 NZ_CP012376:4804555-4804923 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5