Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3163755..3164409 Replicon chromosome
Accession NZ_CP012376
Organism Escherichia coli strain GE3

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AKN41_RS15465 Protein ID WP_000244772.1
Coordinates 3163755..3164162 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AKN41_RS15470 Protein ID WP_000354046.1
Coordinates 3164143..3164409 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKN41_RS15445 3159712..3161445 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
AKN41_RS15450 3161451..3162161 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AKN41_RS15455 3162186..3163082 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AKN41_RS15460 3163194..3163715 + 522 WP_001055874.1 flavodoxin FldB -
AKN41_RS15465 3163755..3164162 - 408 WP_000244772.1 protein YgfX Toxin
AKN41_RS15470 3164143..3164409 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AKN41_RS15475 3164652..3165632 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AKN41_RS15480 3165709..3166368 - 660 WP_000250274.1 hemolysin III family protein -
AKN41_RS15485 3166532..3166843 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
AKN41_RS15490 3166888..3168321 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
AKN41_RS15495 3168378..3169121 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T56348 WP_000244772.1 NZ_CP012376:c3164162-3163755 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T56348 NZ_CP012376:c3164162-3163755 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT56348 WP_000354046.1 NZ_CP012376:c3164409-3164143 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT56348 NZ_CP012376:c3164409-3164143 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References