Detailed information of TA system TA056346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2716456..2717081
• Replicon: chromosome
• Accession: NZ_CP012376
• Organism: Escherichia coli strain GE3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AKN41_RS13300
• Protein ID: WP_000911330.1
• Coordinates: 2716456..2716854 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AKN41_RS13305
• Protein ID: WP_000450524.1
• Coordinates: 2716854..2717081 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKN41_RS13280	2712335..2712535	+	201	WP_000383836.1	YpfN family protein	-
AKN41_RS13285	2712644..2713342	-	699	WP_000679823.1	esterase	-
AKN41_RS13290	2713416..2715431	-	2016	WP_149032399.1	tRNA cytosine(34) acetyltransferase TmcA	-
AKN41_RS13295	2715446..2716309	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
AKN41_RS13300	2716456..2716854	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AKN41_RS13305	2716854..2717081	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AKN41_RS13310	2717235..2717948	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AKN41_RS13315	2718161..2719195	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
AKN41_RS13320	2719212..2720090	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AKN41_RS13325	2720236..2720808	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
AKN41_RS13330	2720808..2721278	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T56346 WP_000911330.1 NZ_CP012376:c2716854-2716456 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T56346 NZ_CP012376:c2716854-2716456 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT56346 WP_000450524.1 NZ_CP012376:c2717081-2716854 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT56346 NZ_CP012376:c2717081-2716854 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33