Detailed information of TA system TA056331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3468977..3469548
• Replicon: chromosome
• Accession: NZ_CP012375
• Organism: Microcystis aeruginosa NIES-2481

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: amyaer_RS17120
• Protein ID: WP_046662963.1
• Coordinates: 3469216..3469548 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I4FQ39
• Locus tag: amyaer_RS17115
• Protein ID: WP_002760402.1
• Coordinates: 3468977..3469219 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
amyaer_RS22110	3464333..3464476	+	144	WP_158524809.1	hypothetical protein	-
amyaer_RS17085	3464486..3464947	-	462	WP_002800691.1	hypothetical protein	-
amyaer_RS22115	3465039..3465194	+	156	WP_158524810.1	hypothetical protein	-
amyaer_RS17090	3465249..3465644	-	396	Protein_3247	helix-turn-helix domain-containing protein	-
amyaer_RS17095	3465659..3466353	+	695	WP_103672892.1	IS1 family transposase	-
amyaer_RS17100	3466377..3466982	-	606	Protein_3249	AraC family transcriptional regulator	-
amyaer_RS17105	3467413..3468075	+	663	WP_002780534.1	potassium channel family protein	-
amyaer_RS17110	3468109..3468885	+	777	WP_046662962.1	DUF2092 domain-containing protein	-
amyaer_RS17115	3468977..3469219	+	243	WP_002760402.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
amyaer_RS17120	3469216..3469548	+	333	WP_046662963.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
amyaer_RS17130	3469907..3470800	+	894	WP_046662964.1	hypothetical protein	-
amyaer_RS17135	3470797..3473859	-	3063	WP_066030075.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12596.67 Da
• Isoelectric Point: 10.1581

>T56331 WP_046662963.1 NZ_CP012375:3469216-3469548 [Microcystis aeruginosa NIES-2481]
VTYIPERGDFVRLSFDPQIGHEQMGNRPALVVSPIDFNRKIGFAFVCPVSNTQRQNPFYVKIPDGEAVTGVIMVDQLRSL
DFRARKASFIGKCPERLLQDVFRRIKPILF

Nucleotide

• Length: 333 bp

>T56331 NZ_CP012375:3469216-3469548 [Microcystis aeruginosa NIES-2481]
GTGACATACATTCCAGAACGTGGAGATTTCGTGCGTTTGAGCTTTGACCCACAAATTGGGCATGAGCAAATGGGTAATCG
TCCAGCACTGGTGGTAAGCCCTATTGATTTTAATCGCAAGATTGGTTTTGCCTTTGTCTGCCCGGTGAGCAATACGCAAC
GCCAGAACCCTTTCTACGTCAAGATTCCAGATGGAGAGGCGGTGACAGGAGTTATCATGGTAGATCAACTGCGATCGTTG
GACTTCCGAGCGAGAAAAGCGAGCTTTATCGGCAAGTGTCCAGAACGGTTGCTCCAGGATGTTTTCAGAAGAATTAAGCC
GATTCTGTTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8956.30 Da
• Isoelectric Point: 4.0460

>AT56331 WP_002760402.1 NZ_CP012375:3468977-3469219 [Microcystis aeruginosa NIES-2481]
MKSQIGRWGNSLAIRIPKYAVEALNLNPKDVVECSVENGKLVIELIQALPELSLEELLAEVVESPEPEVDWGRPMGEEVW

Nucleotide

• Length: 243 bp

>AT56331 NZ_CP012375:3468977-3469219 [Microcystis aeruginosa NIES-2481]
ATGAAGTCGCAGATTGGACGCTGGGGTAATTCTTTAGCGATTCGGATTCCTAAGTATGCGGTGGAGGCGCTGAACCTCAA
TCCTAAGGACGTTGTTGAATGTTCTGTGGAAAACGGAAAGCTAGTAATTGAGCTAATTCAAGCCTTGCCTGAGTTAAGCC
TAGAAGAACTACTGGCTGAGGTTGTTGAATCACCGGAGCCGGAAGTGGATTGGGGACGGCCAATGGGAGAGGAGGTTTGG
TGA

Structures

Antitoxin

• AlphaFold DB: I4FQ39