Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3349004..3349785 Replicon chromosome
Accession NZ_CP012375
Organism Microcystis aeruginosa NIES-2481

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A0F6U717
Locus tag amyaer_RS16475 Protein ID WP_046662887.1
Coordinates 3349291..3349785 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID I4I6J8
Locus tag amyaer_RS16470 Protein ID WP_002799472.1
Coordinates 3349004..3349294 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
amyaer_RS16445 3344834..3345148 - 315 WP_046662882.1 hypothetical protein -
amyaer_RS22510 3345142..3345438 - 297 WP_046662883.1 hypothetical protein -
amyaer_RS16455 3345520..3346608 - 1089 WP_046662884.1 amidohydrolase -
amyaer_RS16460 3347072..3347650 + 579 WP_046662885.1 hypothetical protein -
amyaer_RS16465 3347788..3348690 + 903 WP_046662886.1 DUF1350 family protein -
amyaer_RS16470 3349004..3349294 + 291 WP_002799472.1 DUF1778 domain-containing protein Antitoxin
amyaer_RS16475 3349291..3349785 + 495 WP_046662887.1 GNAT family N-acetyltransferase Toxin
amyaer_RS16480 3349937..3350167 - 231 WP_046662888.1 hypothetical protein -
amyaer_RS16485 3350164..3350538 - 375 WP_002757633.1 nucleotidyltransferase domain-containing protein -
amyaer_RS16490 3351241..3351393 + 153 WP_080949777.1 DUF4160 domain-containing protein -
amyaer_RS16495 3351495..3351839 + 345 WP_004163940.1 type II toxin-antitoxin system RelE/ParE family toxin -
amyaer_RS16500 3351842..3352141 + 300 WP_012266232.1 helix-turn-helix domain-containing protein -
amyaer_RS16505 3352176..3354473 - 2298 WP_046662889.1 TOMM precursor leader peptide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-142)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 17704.36 Da        Isoelectric Point: 8.1174

>T56330 WP_046662887.1 NZ_CP012375:3349291-3349785 [Microcystis aeruginosa NIES-2481]
MNLTPPEPLASYHSITDFSCGITSLDDWLKRRAYANQASGATRTFVTCVDNRVVGYYAFASGAISIQSANGKFRRNMPNP
IPVVILARLAVDTSYQGQGLGCGLFRDGALRVVKAADTIGSRGIIVHAISEEAKNFYLALGFDVSPLEPMTLMITLNDLR
ACIA

Download         Length: 495 bp

>T56330 NZ_CP012375:3349291-3349785 [Microcystis aeruginosa NIES-2481]
ATGAATTTGACACCACCTGAACCTCTGGCTAGTTATCACTCAATCACTGATTTTTCCTGCGGAATTACTTCTCTTGATGA
TTGGTTGAAGCGTCGAGCTTATGCTAATCAAGCCAGTGGTGCAACTAGAACTTTTGTTACCTGCGTTGACAATAGGGTAG
TTGGGTATTACGCCTTTGCTTCCGGGGCGATTAGTATACAATCAGCTAATGGTAAATTTCGGCGGAATATGCCTAACCCA
ATTCCAGTGGTAATTTTGGCACGATTGGCAGTAGATACTTCTTACCAAGGTCAAGGTTTAGGATGCGGTTTATTTCGTGA
TGGTGCGCTACGGGTTGTTAAAGCGGCTGATACCATTGGTAGTCGGGGAATTATTGTTCATGCTATTTCAGAAGAAGCTA
AGAATTTTTATTTGGCTTTAGGTTTTGATGTGTCTCCTCTTGAACCGATGACGTTGATGATTACTCTTAATGATTTACGC
GCTTGTATTGCTTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10968.61 Da        Isoelectric Point: 9.7643

>AT56330 WP_002799472.1 NZ_CP012375:3349004-3349294 [Microcystis aeruginosa NIES-2481]
MSSKTKSKTPRNTLNLRIKPEERNLIDMAAKVQGKNRTDFILEAARNAAEETLLERTIFLASPEAYAEFIALLDAPPQPN
ERLRKTMQTLAPWEKE

Download         Length: 291 bp

>AT56330 NZ_CP012375:3349004-3349294 [Microcystis aeruginosa NIES-2481]
ATGAGTAGTAAAACAAAAAGTAAGACTCCGCGTAATACCCTAAATTTGCGAATTAAGCCAGAAGAACGCAATTTGATCGA
TATGGCAGCCAAAGTGCAAGGTAAGAATAGAACTGATTTTATTTTGGAAGCCGCGCGGAATGCTGCGGAGGAAACTTTGC
TCGAACGTACTATTTTTTTGGCTAGTCCAGAGGCTTATGCAGAATTTATCGCACTTCTAGATGCACCACCTCAACCTAAT
GAACGTTTACGCAAAACCATGCAGACGCTCGCACCTTGGGAGAAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6U717

Antitoxin

Source ID Structure
AlphaFold DB I4I6J8

References