Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/COG3657-dnstrm_HI1420
Location 1632790..1633380 Replicon chromosome
Accession NZ_CP012375
Organism Microcystis aeruginosa NIES-2481

Toxin (Protein)


Gene name PumA Uniprot ID A0A0F6RKM7
Locus tag amyaer_RS07830 Protein ID WP_046661678.1
Coordinates 1633090..1633380 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I4ID81
Locus tag amyaer_RS07825 Protein ID WP_002757266.1
Coordinates 1632790..1633080 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
amyaer_RS07800 1628724..1630322 - 1599 WP_046661674.1 carboxylate--amine ligase -
amyaer_RS07805 1630488..1630616 - 129 WP_002738148.1 photosystem I reaction center subunit IX -
amyaer_RS07810 1630662..1631156 - 495 WP_002748906.1 photosystem I reaction center subunit III -
amyaer_RS07815 1631289..1631708 - 420 WP_002757262.1 secondary thiamine-phosphate synthase enzyme YjbQ -
amyaer_RS07820 1631719..1632519 - 801 WP_046661676.1 uroporphyrinogen-III C-methyltransferase -
amyaer_RS07825 1632790..1633080 - 291 WP_002757266.1 putative addiction module antidote protein Antitoxin
amyaer_RS07830 1633090..1633380 - 291 WP_046661678.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
amyaer_RS07835 1633558..1634364 - 807 WP_052734169.1 matrixin family metalloprotease -
amyaer_RS07840 1634575..1635063 + 489 WP_046661680.1 hypothetical protein -
amyaer_RS07845 1635209..1635499 + 291 WP_002757283.1 nucleotidyltransferase -
amyaer_RS07850 1635489..1635839 + 351 WP_046661681.1 DUF86 domain-containing protein -
amyaer_RS07855 1636220..1637119 + 900 WP_046661682.1 agmatinase -
amyaer_RS07865 1637301..1638350 + 1050 WP_046661683.1 aromatic ring-hydroxylating dioxygenase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Uncharacterized protein HI1420

(7-92)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11054.65 Da        Isoelectric Point: 10.1314

>T56325 WP_046661678.1 NZ_CP012375:c1633380-1633090 [Microcystis aeruginosa NIES-2481]
MIEIRQTETYSQWFSNLRDRQAKARIDIRVRRLSMGNPGDVKPVGKGVSELRIDYGQGYRVYFIQRGETLIILLAAGDKQ
TQERDIKTALNLAQDL

Download         Length: 291 bp

>T56325 NZ_CP012375:c1633380-1633090 [Microcystis aeruginosa NIES-2481]
ATGATTGAAATTCGCCAGACCGAAACCTACTCTCAGTGGTTTAGCAATTTGCGGGATCGCCAAGCAAAAGCACGTATAGA
CATTCGTGTTCGTCGCCTGTCTATGGGCAATCCAGGCGATGTTAAACCAGTAGGAAAAGGTGTATCGGAACTGCGGATCG
ATTATGGCCAAGGCTATCGGGTGTATTTCATTCAGCGAGGAGAGACCTTGATCATCCTCTTGGCAGCAGGAGATAAGCAA
ACTCAAGAACGGGACATCAAAACAGCATTAAATTTAGCACAAGACTTATAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10320.80 Da        Isoelectric Point: 4.4475

>AT56325 WP_002757266.1 NZ_CP012375:c1633080-1632790 [Microcystis aeruginosa NIES-2481]
MATIQTYPWDAAEHLETKEDIAAYLEAALEDGDPSLVVAALGDIARSKGMTHIARETGLGRESLYKALSIEGNPEFATVL
KVLQSLGLRLQVVPIA

Download         Length: 291 bp

>AT56325 NZ_CP012375:c1633080-1632790 [Microcystis aeruginosa NIES-2481]
ATGGCTACTATCCAAACCTATCCTTGGGATGCGGCAGAACATCTCGAAACAAAAGAAGATATAGCTGCATATCTTGAAGC
AGCTCTTGAAGATGGCGATCCAAGCTTAGTGGTAGCAGCATTAGGTGATATTGCTCGATCTAAAGGGATGACACATATTG
CCCGTGAAACAGGGTTGGGGCGTGAAAGTCTCTATAAAGCTTTGTCAATCGAAGGCAATCCAGAGTTTGCCACAGTTCTT
AAGGTCTTACAATCACTTGGGTTACGCCTCCAAGTCGTACCAATTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6RKM7

Antitoxin

Source ID Structure
AlphaFold DB I4ID81

References