Detailed information of TA system TA056317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2260633..2261239
• Replicon: chromosome
• Accession: NZ_CP012370
• Organism: Moorella thermoacetica strain DSM 2955

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1D7XDY8
• Locus tag: MOTHA_RS11190
• Protein ID: WP_011393652.1
• Coordinates: 2260633..2260983 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MOTHA_RS11195
• Protein ID: WP_036371414.1
• Coordinates: 2260985..2261239 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTHA_RS11155	2256243..2256728	-	486	WP_036371410.1	ribosomal protein S18-alanine N-acetyltransferase	-
MOTHA_RS11160	2256746..2257432	-	687	WP_011393646.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
MOTHA_RS11165	2257413..2257880	-	468	WP_011393647.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
MOTHA_RS11170	2258037..2258615	-	579	WP_200901660.1	uracil-DNA glycosylase	-
MOTHA_RS11175	2258667..2259299	-	633	WP_011393649.1	thiamine phosphate synthase	-
MOTHA_RS11180	2259380..2259769	-	390	WP_025773384.1	response regulator	-
MOTHA_RS11185	2259918..2260619	-	702	WP_011393651.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
MOTHA_RS11190	2260633..2260983	-	351	WP_011393652.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MOTHA_RS11195	2260985..2261239	-	255	WP_036371414.1	ribbon-helix-helix protein, CopG family	Antitoxin
MOTHA_RS11200	2261367..2262488	-	1122	WP_025773385.1	alanine racemase	-
MOTHA_RS11205	2262460..2264052	-	1593	WP_011393655.1	NAD(P)H-hydrate dehydratase	-
MOTHA_RS11210	2264057..2264443	-	387	WP_011393656.1	holo-ACP synthase	-
MOTHA_RS11215	2264794..2265783	+	990	WP_011393657.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12684.71 Da
• Isoelectric Point: 7.2034

>T56317 WP_011393652.1 NZ_CP012370:c2260983-2260633 [Moorella thermoacetica]
MLVRRGDVFYAHLNPVVGSEQGGTRPVLIIQNDIGNQYSPTTIVAAITSQIAKAKLPTHVEISAAKSGLERDSVILLEQI
RTIDKSRLKQKVAVLDDEILEKVNRALEISLGLVEV

Nucleotide

• Length: 351 bp

>T56317 NZ_CP012370:c2260983-2260633 [Moorella thermoacetica]
ATGCTGGTACGGCGCGGGGATGTCTTTTACGCCCATCTCAATCCGGTGGTCGGTTCCGAGCAAGGCGGAACCCGCCCGGT
ACTTATCATTCAAAATGACATTGGCAACCAGTATAGCCCCACTACTATTGTGGCCGCTATTACTTCCCAGATTGCCAAGG
CCAAACTGCCCACCCATGTGGAAATCAGCGCTGCCAAGAGCGGCCTGGAGCGCGATTCTGTTATTCTGCTGGAACAGATC
CGCACCATAGACAAGAGCCGCCTCAAACAAAAGGTGGCCGTCCTGGATGATGAGATCCTGGAAAAGGTGAACCGGGCCCT
GGAAATAAGCCTGGGACTGGTGGAAGTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9983.45 Da
• Isoelectric Point: 6.3653

>AT56317 WP_036371414.1 NZ_CP012370:c2261239-2260985 [Moorella thermoacetica]
MISLPESLLAEVDGLANLEKRNRSEFIREAMKLYITERKRRNIREQMKRGYQEMAPINLALAVENYDVENEVQRYYDKSL
AECK

Nucleotide

• Length: 255 bp

>AT56317 NZ_CP012370:c2261239-2260985 [Moorella thermoacetica]
ATGATCAGCTTGCCGGAGAGCCTCCTGGCCGAGGTTGACGGCCTGGCGAACCTTGAAAAGCGTAACCGCAGCGAGTTTAT
CCGGGAAGCTATGAAACTATATATTACCGAGCGTAAAAGGCGCAATATCCGGGAACAAATGAAACGGGGATACCAGGAAA
TGGCACCTATCAATTTAGCCCTTGCAGTGGAAAACTATGATGTAGAGAATGAAGTACAAAGATACTACGATAAAAGCCTG
GCGGAGTGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A1D7XDY8