Detailed information of TA system TA056315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2164848..2165454
• Replicon: chromosome
• Accession: NZ_CP012369
• Organism: Moorella thermoacetica strain DSM 521

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1D7XDY8
• Locus tag: MOTHE_RS10735
• Protein ID: WP_011393652.1
• Coordinates: 2164848..2165198 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MOTHE_RS10740
• Protein ID: WP_036371414.1
• Coordinates: 2165200..2165454 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTHE_RS10700	2160458..2160943	-	486	WP_036371410.1	ribosomal protein S18-alanine N-acetyltransferase	-
MOTHE_RS10705	2160961..2161647	-	687	WP_011393646.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
MOTHE_RS10710	2161628..2162095	-	468	WP_011393647.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
MOTHE_RS10715	2162252..2162830	-	579	WP_200901660.1	uracil-DNA glycosylase	-
MOTHE_RS10720	2162882..2163514	-	633	WP_011393649.1	thiamine phosphate synthase	-
MOTHE_RS10725	2163595..2163777	-	183	WP_053095107.1	response regulator	-
MOTHE_RS12965	2163799..2163984	-	186	WP_011393650.1	response regulator	-
MOTHE_RS10730	2164133..2164834	-	702	WP_011393651.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
MOTHE_RS10735	2164848..2165198	-	351	WP_011393652.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MOTHE_RS10740	2165200..2165454	-	255	WP_036371414.1	ribbon-helix-helix protein, CopG family	Antitoxin
MOTHE_RS10745	2165582..2166703	-	1122	WP_053095108.1	alanine racemase	-
MOTHE_RS10750	2166675..2168267	-	1593	WP_011393655.1	NAD(P)H-hydrate dehydratase	-
MOTHE_RS10755	2168272..2168658	-	387	WP_011393656.1	holo-ACP synthase	-
MOTHE_RS10760	2169009..2169998	+	990	WP_011393657.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12684.71 Da
• Isoelectric Point: 7.2034

>T56315 WP_011393652.1 NZ_CP012369:c2165198-2164848 [Moorella thermoacetica]
MLVRRGDVFYAHLNPVVGSEQGGTRPVLIIQNDIGNQYSPTTIVAAITSQIAKAKLPTHVEISAAKSGLERDSVILLEQI
RTIDKSRLKQKVAVLDDEILEKVNRALEISLGLVEV

Nucleotide

• Length: 351 bp

>T56315 NZ_CP012369:c2165198-2164848 [Moorella thermoacetica]
ATGCTGGTACGGCGCGGGGATGTCTTTTACGCCCATCTCAATCCGGTGGTCGGTTCCGAGCAAGGCGGAACCCGCCCGGT
ACTTATCATTCAAAATGACATTGGCAACCAGTATAGCCCCACTACTATTGTGGCCGCTATTACTTCCCAGATTGCCAAGG
CCAAACTGCCCACCCATGTGGAAATCAGCGCTGCCAAGAGCGGCCTGGAGCGCGATTCTGTTATTCTGCTGGAACAGATC
CGCACCATAGACAAGAGCCGCCTCAAACAAAAGGTGGCCGTCCTGGATGATGAGATCCTGGAAAAGGTGAACCGGGCCCT
GGAAATAAGCCTGGGACTGGTGGAAGTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9983.45 Da
• Isoelectric Point: 6.3653

>AT56315 WP_036371414.1 NZ_CP012369:c2165454-2165200 [Moorella thermoacetica]
MISLPESLLAEVDGLANLEKRNRSEFIREAMKLYITERKRRNIREQMKRGYQEMAPINLALAVENYDVENEVQRYYDKSL
AECK

Nucleotide

• Length: 255 bp

>AT56315 NZ_CP012369:c2165454-2165200 [Moorella thermoacetica]
ATGATCAGCTTGCCGGAGAGCCTCCTGGCCGAGGTTGACGGCCTGGCGAACCTTGAAAAGCGTAACCGCAGCGAGTTTAT
CCGGGAAGCTATGAAACTATATATTACCGAGCGTAAAAGGCGCAATATCCGGGAACAAATGAAACGGGGATACCAGGAAA
TGGCACCTATCAATTTAGCCCTTGCAGTGGAAAACTATGATGTAGAGAATGAAGTACAAAGATACTACGATAAAAGCCTG
GCGGAGTGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A1D7XDY8