Detailed information of TA system TA056313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 11359..12496
• Replicon: plasmid unnamed 2
• Accession: NZ_CP012368
• Organism: Enterococcus durans strain KLDS 6.0933

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: LIANG_RS14510
• Protein ID: WP_016177608.1
• Coordinates: 11359..12222 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: LIANG_RS14515
• Protein ID: WP_000301765.1
• Coordinates: 12224..12496 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIANG_RS14475 (LIANG_14490)	6412..7296	-	885	WP_053109524.1	reverse transcriptase family protein	-
LIANG_RS14480 (LIANG_14495)	7299..7580	-	282	WP_002337516.1	helix-turn-helix transcriptional regulator	-
LIANG_RS15450	7627..7785	-	159	WP_002337517.1	hypothetical protein	-
LIANG_RS15290	8285..8419	-	135	Protein_9	IS256 family transposase	-
LIANG_RS15455	8541..8717	+	177	WP_016629480.1	hypothetical protein	-
LIANG_RS14485 (LIANG_14500)	8720..8977	+	258	WP_016629479.1	hypothetical protein	-
LIANG_RS14490 (LIANG_14505)	9000..9233	-	234	WP_016629478.1	hypothetical protein	-
LIANG_RS14495 (LIANG_14510)	9338..9835	-	498	WP_025481726.1	molecular chaperone DnaJ	-
LIANG_RS14500 (LIANG_14515)	10368..10685	-	318	WP_002300567.1	hypothetical protein	-
LIANG_RS14505 (LIANG_14520)	11119..11331	-	213	WP_053111889.1	hypothetical protein	-
LIANG_RS14510 (LIANG_14525)	11359..12222	-	864	WP_016177608.1	zeta toxin family protein	Toxin
LIANG_RS14515 (LIANG_14530)	12224..12496	-	273	WP_000301765.1	antitoxin	Antitoxin
LIANG_RS14520 (LIANG_14535)	12513..12728	-	216	WP_002295735.1	peptide-binding protein	-
LIANG_RS14525 (LIANG_14540)	12820..13632	-	813	WP_220715876.1	ParA family protein	-
LIANG_RS14530 (LIANG_14545)	13778..13954	-	177	Protein_20	topoisomerase	-
LIANG_RS14535 (LIANG_14550)	14031..14711	+	681	WP_053109531.1	IS6-like element IS1216 family transposase	-
LIANG_RS14540 (LIANG_14555)	14806..16335	+	1530	WP_002337537.1	multicopper oxidase domain-containing protein	-
LIANG_RS14545 (LIANG_14560)	16450..16584	-	135	WP_105195096.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..41490	41490

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32477.05 Da
• Isoelectric Point: 6.6610

>T56313 WP_016177608.1 NZ_CP012368:c12222-11359 [Enterococcus durans]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKIPKLPGL

Nucleotide

• Length: 864 bp

>T56313 NZ_CP012368:c12222-11359 [Enterococcus durans]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACACCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCTAAAATACCTAAACTTCCAGGTCTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT56313 WP_000301765.1 NZ_CP012368:c12496-12224 [Enterococcus durans]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT56313 NZ_CP012368:c12496-12224 [Enterococcus durans]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3