Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 11359..12496 Replicon plasmid unnamed 2
Accession NZ_CP012368
Organism Enterococcus durans strain KLDS 6.0933

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag LIANG_RS14510 Protein ID WP_016177608.1
Coordinates 11359..12222 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LIANG_RS14515 Protein ID WP_000301765.1
Coordinates 12224..12496 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIANG_RS14475 (LIANG_14490) 6412..7296 - 885 WP_053109524.1 reverse transcriptase family protein -
LIANG_RS14480 (LIANG_14495) 7299..7580 - 282 WP_002337516.1 helix-turn-helix transcriptional regulator -
LIANG_RS15450 7627..7785 - 159 WP_002337517.1 hypothetical protein -
LIANG_RS15290 8285..8419 - 135 Protein_9 IS256 family transposase -
LIANG_RS15455 8541..8717 + 177 WP_016629480.1 hypothetical protein -
LIANG_RS14485 (LIANG_14500) 8720..8977 + 258 WP_016629479.1 hypothetical protein -
LIANG_RS14490 (LIANG_14505) 9000..9233 - 234 WP_016629478.1 hypothetical protein -
LIANG_RS14495 (LIANG_14510) 9338..9835 - 498 WP_025481726.1 molecular chaperone DnaJ -
LIANG_RS14500 (LIANG_14515) 10368..10685 - 318 WP_002300567.1 hypothetical protein -
LIANG_RS14505 (LIANG_14520) 11119..11331 - 213 WP_053111889.1 hypothetical protein -
LIANG_RS14510 (LIANG_14525) 11359..12222 - 864 WP_016177608.1 zeta toxin family protein Toxin
LIANG_RS14515 (LIANG_14530) 12224..12496 - 273 WP_000301765.1 antitoxin Antitoxin
LIANG_RS14520 (LIANG_14535) 12513..12728 - 216 WP_002295735.1 peptide-binding protein -
LIANG_RS14525 (LIANG_14540) 12820..13632 - 813 WP_220715876.1 ParA family protein -
LIANG_RS14530 (LIANG_14545) 13778..13954 - 177 Protein_20 topoisomerase -
LIANG_RS14535 (LIANG_14550) 14031..14711 + 681 WP_053109531.1 IS6-like element IS1216 family transposase -
LIANG_RS14540 (LIANG_14555) 14806..16335 + 1530 WP_002337537.1 multicopper oxidase domain-containing protein -
LIANG_RS14545 (LIANG_14560) 16450..16584 - 135 WP_105195096.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..41490 41490


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32477.05 Da        Isoelectric Point: 6.6610

>T56313 WP_016177608.1 NZ_CP012368:c12222-11359 [Enterococcus durans]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKIPKLPGL

Download         Length: 864 bp

>T56313 NZ_CP012368:c12222-11359 [Enterococcus durans]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACACCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCTAAAATACCTAAACTTCCAGGTCTTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT56313 WP_000301765.1 NZ_CP012368:c12496-12224 [Enterococcus durans]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT56313 NZ_CP012368:c12496-12224 [Enterococcus durans]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References