Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3359875..3360511 Replicon chromosome
Accession NZ_CP012330
Organism Bacillus altitudinis strain NJ-V

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag AKO66_RS17330 Protein ID WP_003214169.1
Coordinates 3359875..3360225 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag AKO66_RS17335 Protein ID WP_003214273.1
Coordinates 3360230..3360511 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO66_RS17290 3354908..3355507 - 600 WP_008346897.1 SpoIIE family protein phosphatase -
AKO66_RS17295 3355507..3356295 - 789 WP_012009000.1 RNA polymerase sigma factor SigB -
AKO66_RS17300 3356261..3356749 - 489 WP_007496471.1 anti-sigma B factor RsbW -
AKO66_RS17305 3356746..3357075 - 330 WP_057078430.1 anti-sigma factor antagonist -
AKO66_RS17310 3357135..3358142 - 1008 WP_048002360.1 PP2C family protein-serine/threonine phosphatase -
AKO66_RS17315 3358153..3358554 - 402 WP_003214085.1 anti-sigma regulatory factor -
AKO66_RS17320 3358557..3358925 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
AKO66_RS17325 3358930..3359760 - 831 WP_017358395.1 STAS domain-containing protein -
AKO66_RS17330 3359875..3360225 - 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AKO66_RS17335 3360230..3360511 - 282 WP_003214273.1 hypothetical protein Antitoxin
AKO66_RS17340 3360805..3361986 - 1182 WP_057078432.1 alanine racemase -
AKO66_RS17345 3362115..3363131 - 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
AKO66_RS17350 3363292..3363657 - 366 WP_017358398.1 holo-ACP synthase -
AKO66_RS17355 3363752..3364357 + 606 WP_007496491.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T56297 WP_003214169.1 NZ_CP012330:c3360225-3359875 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T56297 NZ_CP012330:c3360225-3359875 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT56297 WP_003214273.1 NZ_CP012330:c3360511-3360230 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT56297 NZ_CP012330:c3360511-3360230 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References