Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 323657..324293 Replicon chromosome
Accession NZ_CP012329
Organism Bacillus altitudinis strain NJ-M2

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag AKO65_RS01680 Protein ID WP_003214169.1
Coordinates 323657..324007 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag AKO65_RS01685 Protein ID WP_003214273.1
Coordinates 324012..324293 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO65_RS01640 318690..319289 - 600 WP_008346897.1 SpoIIE family protein phosphatase -
AKO65_RS01645 319289..320077 - 789 WP_012009000.1 RNA polymerase sigma factor SigB -
AKO65_RS01650 320043..320531 - 489 WP_007496471.1 anti-sigma B factor RsbW -
AKO65_RS01655 320528..320857 - 330 WP_057078430.1 anti-sigma factor antagonist -
AKO65_RS01660 320917..321924 - 1008 WP_048002360.1 PP2C family protein-serine/threonine phosphatase -
AKO65_RS01665 321935..322336 - 402 WP_003214085.1 anti-sigma regulatory factor -
AKO65_RS01670 322339..322707 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
AKO65_RS01675 322712..323542 - 831 WP_017358395.1 STAS domain-containing protein -
AKO65_RS01680 323657..324007 - 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AKO65_RS01685 324012..324293 - 282 WP_003214273.1 hypothetical protein Antitoxin
AKO65_RS01690 324587..325768 - 1182 WP_057078432.1 alanine racemase -
AKO65_RS01695 325897..326913 - 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
AKO65_RS01700 327074..327439 - 366 WP_017358398.1 holo-ACP synthase -
AKO65_RS01705 327534..328139 + 606 WP_007496491.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T56296 WP_003214169.1 NZ_CP012329:c324007-323657 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T56296 NZ_CP012329:c324007-323657 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT56296 WP_003214273.1 NZ_CP012329:c324293-324012 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT56296 NZ_CP012329:c324293-324012 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References