Detailed information of TA system TA056240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-vapB/MazF(toxin)
• Location: 1433643..1434464
• Replicon: chromosome
• Accession: NZ_CP012294
• Organism: Pediococcus damnosus strain TMW 2.1536

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: ADU73_RS06685
• Protein ID: WP_062913313.1
• Coordinates: 1433643..1434014 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0R2HM84
• Locus tag: ADU73_RS06690
• Protein ID: WP_046871612.1
• Coordinates: 1434099..1434464 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ADU73_RS06665	1429926..1431488	+	1563	WP_046871607.1	DEAD/DEAH box helicase	-
ADU73_RS06670	1431781..1432140	+	360	WP_046871608.1	holo-ACP synthase	-
ADU73_RS06675	1432147..1433274	+	1128	WP_046871609.1	alanine racemase	-
ADU73_RS06680	1433352..1433624	+	273	WP_141306217.1	hypothetical protein	-
ADU73_RS06685	1433643..1434014	+	372	WP_062913313.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ADU73_RS06690	1434099..1434464	+	366	WP_046871612.1	DUF488 family protein	Antitoxin
ADU73_RS06695	1434461..1434949	-	489	WP_062913314.1	GNAT family N-acetyltransferase	-
ADU73_RS06700	1435044..1435553	+	510	WP_046871614.1	QueT transporter family protein	-
ADU73_RS06705	1435931..1436578	-	648	WP_046871615.1	CBS domain-containing protein	-
ADU73_RS06710	1436912..1438288	-	1377	WP_062913143.1	ISLre2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-113)

Antitoxin

• Protein of unknown function DUF488 (4-115)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13269.33 Da
• Isoelectric Point: 8.5686

>T56240 WP_062913313.1 NZ_CP012294:1433643-1434014 [Pediococcus damnosus]
MSEANVKRGDIFYADLSPVVGSEQGGLRPVLIVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAAETGVERNSVILL
EQIRTIDKQRLKDQVGQLAVSKMLEVDTALKVSVGLAENVQHT

Nucleotide

• Length: 372 bp

>T56240 NZ_CP012294:1433643-1434014 [Pediococcus damnosus]
ATGAGTGAAGCCAACGTTAAACGTGGCGACATTTTTTATGCTGACTTGTCTCCTGTAGTTGGGTCTGAACAGGGTGGTTT
GCGCCCTGTTTTAATTGTCCAAAACAATATTGGTAACCACTATAGTCCTACTGTGATTGTGGCAGCTATTACTGCTAAAA
TACAAAAACCAAAAATGCCAACCCACGTTGGAATTACAGCTGCAGAGACGGGTGTTGAACGAAATTCGGTTATTTTGCTA
GAACAAATTCGAACAATTGATAAACAACGACTTAAAGATCAGGTCGGACAATTGGCTGTTAGCAAAATGCTTGAGGTAGA
TACAGCCTTAAAAGTGAGTGTCGGCCTGGCTGAGAATGTTCAACATACATAG

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 14344.42 Da
• Isoelectric Point: 7.2021

>AT56240 WP_046871612.1 NZ_CP012294:1434099-1434464 [Pediococcus damnosus]
MEIKLERIYTKPADLSGYRVLVDRLWPRGISKVNAQLDWWAKEIGPSTELRKWFDHDPKKFEEFSKKYETELNTAEFSTE
FMTQIKKQLQTQDVIFLYGAKDEKHNQAVILKTYVEAKLAD

Nucleotide

• Length: 366 bp

>AT56240 NZ_CP012294:1434099-1434464 [Pediococcus damnosus]
ATGGAAATTAAGTTGGAACGTATTTATACAAAGCCCGCAGATTTATCGGGATATCGTGTCTTGGTTGACCGATTATGGCC
ACGGGGGATTTCTAAGGTTAACGCTCAGTTAGATTGGTGGGCAAAAGAAATAGGACCAAGTACCGAACTAAGAAAGTGGT
TCGATCATGATCCTAAAAAGTTTGAGGAATTTTCCAAAAAGTATGAAACAGAACTTAACACAGCAGAATTTAGTACGGAA
TTTATGACACAGATTAAAAAGCAACTGCAGACCCAAGACGTAATTTTTTTGTACGGGGCTAAAGACGAAAAACATAACCA
AGCAGTTATTTTGAAAACGTACGTTGAAGCTAAACTTGCAGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A0R2HM84