Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 3674..4275 Replicon plasmid pL21535-1
Accession NZ_CP012289
Organism Pediococcus damnosus strain TMW 2.1535

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag ADU72_RS10530 Protein ID WP_001748110.1
Coordinates 3674..4018 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag ADU72_RS10535 Protein ID WP_003643337.1
Coordinates 4012..4275 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADU72_RS10515 902..1459 - 558 WP_158509302.1 IS3 family transposase -
ADU72_RS12315 1500..1661 - 162 WP_158510062.1 hypothetical protein -
ADU72_RS10520 1858..3105 + 1248 WP_062904581.1 ISL3 family transposase -
ADU72_RS10530 3674..4018 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ADU72_RS10535 4012..4275 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ADU72_RS10540 4361..4948 + 588 WP_062904616.1 site-specific integrase -
ADU72_RS10545 5402..5701 + 300 Protein_7 helix-turn-helix domain-containing protein -
ADU72_RS10550 5764..6378 + 615 WP_052694451.1 NUMOD4 motif-containing HNH endonuclease -
ADU72_RS10555 6576..7034 + 459 WP_062904617.1 phage terminase small subunit P27 family -
ADU72_RS12320 7048..7200 - 153 WP_155386924.1 hypothetical protein -
ADU72_RS10560 7187..7606 - 420 WP_046870702.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..21309 21309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T56238 WP_001748110.1 NZ_CP012289:c4018-3674 [Pediococcus damnosus]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T56238 NZ_CP012289:c4018-3674 [Pediococcus damnosus]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT56238 WP_003643337.1 NZ_CP012289:c4275-4012 [Pediococcus damnosus]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT56238 NZ_CP012289:c4275-4012 [Pediococcus damnosus]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References