56237

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-vapB/MazF(toxin)
Location 1436460..1437281 Replicon chromosome
Accession NZ_CP012288
Organism Pediococcus damnosus strain TMW 2.1535

Toxin (Protein)


Gene name pemK Uniprot ID A0A0R2HKP8
Locus tag ADU72_RS06615 Protein ID WP_046871611.1
Coordinates 1436460..1436831 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0R2HM84
Locus tag ADU72_RS06620 Protein ID WP_046871612.1
Coordinates 1436916..1437281 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADU72_RS06595 1432743..1434305 + 1563 WP_046871607.1 DEAD/DEAH box helicase -
ADU72_RS06600 1434598..1434957 + 360 WP_046871608.1 holo-ACP synthase -
ADU72_RS06605 1434964..1436091 + 1128 WP_046871609.1 alanine racemase -
ADU72_RS06610 1436169..1436441 + 273 WP_141306217.1 hypothetical protein -
ADU72_RS06615 1436460..1436831 + 372 WP_046871611.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ADU72_RS06620 1436916..1437281 + 366 WP_046871612.1 DUF488 family protein Antitoxin
ADU72_RS06625 1437278..1437766 - 489 WP_046871613.1 GNAT family N-acetyltransferase -
ADU72_RS06630 1437861..1438370 + 510 WP_046871614.1 QueT transporter family protein -
ADU72_RS06635 1438748..1439395 - 648 WP_062904477.1 CBS domain-containing protein -
ADU72_RS06640 1439528..1440919 - 1392 WP_046871616.1 L,D-transpeptidase/peptidoglycan binding protein -
ADU72_RS06645 1441103..1442065 - 963 WP_046871617.1 L-lactate dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-113)

Antitoxin

Protein of unknown function DUF488

(4-115)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13241.28 Da        Isoelectric Point: 8.5686

>T56237 WP_046871611.1 NZ_CP012288:1436460-1436831 [Pediococcus damnosus]
MSEANVKRGDIFYADLSPVVGSEQGGLRPVLIVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAAETGVERNSVILL
EQIRTIDKQRLKDQVGQLAASKMLEVDTALKVSVGLAENVQHT

Download         Length: 372 bp

>T56237 NZ_CP012288:1436460-1436831 [Pediococcus damnosus]
ATGAGTGAAGCCAACGTTAAACGTGGCGACATTTTTTATGCTGACTTGTCTCCTGTAGTTGGGTCTGAACAGGGTGGTTT
GCGCCCTGTTTTAATTGTCCAAAACAATATTGGTAACCACTATAGTCCTACTGTGATTGTGGCAGCTATTACTGCTAAAA
TACAAAAACCAAAAATGCCAACCCACGTTGGAATTACAGCTGCAGAGACGGGTGTTGAACGAAATTCGGTTATTTTGCTA
GAACAAATTCGAACAATTGATAAACAACGACTTAAAGATCAGGTCGGACAATTGGCTGCTAGCAAAATGCTTGAGGTAGA
TACAGCCTTAAAAGTGAGTGTCGGCCTGGCTGAGAATGTTCAACATACATAG

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 14344.42 Da        Isoelectric Point: 7.2021

>AT56237 WP_046871612.1 NZ_CP012288:1436916-1437281 [Pediococcus damnosus]
MEIKLERIYTKPADLSGYRVLVDRLWPRGISKVNAQLDWWAKEIGPSTELRKWFDHDPKKFEEFSKKYETELNTAEFSTE
FMTQIKKQLQTQDVIFLYGAKDEKHNQAVILKTYVEAKLAD

Download         Length: 366 bp

>AT56237 NZ_CP012288:1436916-1437281 [Pediococcus damnosus]
ATGGAAATTAAGTTGGAACGTATTTATACAAAGCCCGCAGATTTATCGGGATATCGTGTCTTGGTTGACCGATTATGGCC
ACGGGGGATTTCTAAGGTTAACGCTCAGTTAGATTGGTGGGCAAAAGAAATAGGACCAAGTACCGAACTAAGAAAGTGGT
TCGATCATGATCCTAAAAAGTTTGAGGAATTTTCCAAAAAGTATGAAACAGAACTTAACACAGCAGAATTTAGTACGGAA
TTTATGACACAGATTAAAAAGCAACTGCAGACCCAAGACGTAATTTTTTTGTACGGGGCTAAAGACGAAAAACATAACCA
AGCAGTTATTTTGAAAACGTACGTTGAAGCTAAACTTGCAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2HKP8

Antitoxin

Source ID Structure
AlphaFold DB A0A0R2HM84

References