Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 573..1174 Replicon plasmid pL21534-1
Accession NZ_CP012284
Organism Pediococcus damnosus strain TMW 2.1534

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag ADU71_RS10495 Protein ID WP_001748110.1
Coordinates 573..917 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag ADU71_RS10500 Protein ID WP_003643337.1
Coordinates 911..1174 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADU71_RS10495 573..917 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ADU71_RS10500 911..1174 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ADU71_RS10505 1260..1847 + 588 WP_057705806.1 site-specific integrase -
ADU71_RS10510 3132..4067 + 936 WP_062904315.1 replication initiation protein -
ADU71_RS10515 4358..4861 + 504 WP_046870764.1 DUF536 domain-containing protein -
ADU71_RS10520 4883..5332 + 450 Protein_6 site-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11687 11687


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T56234 WP_001748110.1 NZ_CP012284:c917-573 [Pediococcus damnosus]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T56234 NZ_CP012284:c917-573 [Pediococcus damnosus]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACGAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT56234 WP_003643337.1 NZ_CP012284:c1174-911 [Pediococcus damnosus]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT56234 NZ_CP012284:c1174-911 [Pediococcus damnosus]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAAGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References