Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-vapB/MazF(toxin)
Location 1020562..1021383 Replicon chromosome
Accession NZ_CP012275
Organism Pediococcus damnosus strain TMW 2.1533

Toxin (Protein)


Gene name pemK Uniprot ID A0A0R2HKP8
Locus tag ADU70_RS04875 Protein ID WP_046871611.1
Coordinates 1021012..1021383 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0R2HM84
Locus tag ADU70_RS04870 Protein ID WP_046871612.1
Coordinates 1020562..1020927 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADU70_RS04845 1015779..1016741 + 963 WP_046871617.1 L-lactate dehydrogenase -
ADU70_RS04850 1016925..1018316 + 1392 WP_046871616.1 L,D-transpeptidase/peptidoglycan binding protein -
ADU70_RS04855 1018449..1019096 + 648 WP_062904477.1 CBS domain-containing protein -
ADU70_RS04860 1019474..1019983 - 510 WP_046871614.1 QueT transporter family protein -
ADU70_RS04865 1020077..1020565 + 489 WP_046871613.1 GNAT family N-acetyltransferase -
ADU70_RS04870 1020562..1020927 - 366 WP_046871612.1 DUF488 family protein Antitoxin
ADU70_RS04875 1021012..1021383 - 372 WP_046871611.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ADU70_RS04880 1021402..1021674 - 273 WP_141306217.1 hypothetical protein -
ADU70_RS04885 1021752..1022888 - 1137 WP_062904478.1 alanine racemase -
ADU70_RS04890 1022895..1023254 - 360 WP_046871608.1 holo-ACP synthase -
ADU70_RS04895 1023547..1025109 - 1563 WP_062904479.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-113)

Antitoxin

Protein of unknown function DUF488

(4-115)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13241.28 Da        Isoelectric Point: 8.5686

>T56227 WP_046871611.1 NZ_CP012275:c1021383-1021012 [Pediococcus damnosus]
MSEANVKRGDIFYADLSPVVGSEQGGLRPVLIVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAAETGVERNSVILL
EQIRTIDKQRLKDQVGQLAASKMLEVDTALKVSVGLAENVQHT

Download         Length: 372 bp

>T56227 NZ_CP012275:c1021383-1021012 [Pediococcus damnosus]
ATGAGTGAAGCCAACGTTAAACGTGGCGACATTTTTTATGCTGACTTGTCTCCTGTAGTTGGGTCTGAACAGGGTGGTTT
GCGCCCTGTTTTAATTGTCCAAAACAATATTGGTAACCACTATAGTCCTACTGTGATTGTGGCAGCTATTACTGCTAAAA
TACAAAAACCAAAAATGCCAACCCACGTTGGAATTACAGCTGCAGAGACGGGTGTTGAACGAAATTCGGTTATTTTGCTA
GAACAAATTCGAACAATTGATAAACAACGACTTAAAGATCAGGTCGGACAATTGGCTGCTAGCAAAATGCTTGAGGTAGA
TACAGCCTTAAAAGTGAGTGTCGGCCTGGCTGAGAATGTTCAACATACATAG

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 14344.42 Da        Isoelectric Point: 7.2021

>AT56227 WP_046871612.1 NZ_CP012275:c1020927-1020562 [Pediococcus damnosus]
MEIKLERIYTKPADLSGYRVLVDRLWPRGISKVNAQLDWWAKEIGPSTELRKWFDHDPKKFEEFSKKYETELNTAEFSTE
FMTQIKKQLQTQDVIFLYGAKDEKHNQAVILKTYVEAKLAD

Download         Length: 366 bp

>AT56227 NZ_CP012275:c1020927-1020562 [Pediococcus damnosus]
ATGGAAATTAAGTTGGAACGTATTTATACAAAGCCCGCAGATTTATCGGGATATCGTGTCTTGGTTGACCGATTATGGCC
ACGGGGGATTTCTAAGGTTAACGCTCAGTTAGATTGGTGGGCAAAAGAAATAGGACCAAGTACCGAACTAAGAAAGTGGT
TCGATCATGATCCTAAAAAGTTTGAGGAATTTTCCAAAAAGTATGAAACAGAACTTAACACAGCAGAATTTAGTACGGAA
TTTATGACACAGATTAAAAAGCAACTGCAGACCCAAGACGTAATTTTTTTGTACGGGGCTAAAGACGAAAAACATAACCA
AGCAGTTATTTTGAAAACGTACGTTGAAGCTAAACTTGCAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2HKP8

Antitoxin

Source ID Structure
AlphaFold DB A0A0R2HM84

References