Detailed information of TA system TA056225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 573..1174
• Replicon: plasmid pL21532-1
• Accession: NZ_CP012270
• Organism: Pediococcus damnosus strain TMW 2.1532

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: ADU69_RS09975
• Protein ID: WP_001748110.1
• Coordinates: 573..917 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: Q684P2
• Locus tag: ADU69_RS09980
• Protein ID: WP_003643337.1
• Coordinates: 911..1174 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ADU69_RS09975	573..917	-	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ADU69_RS09980	911..1174	-	264	WP_003643337.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ADU69_RS09985	1260..1847	+	588	WP_057705806.1	site-specific integrase	-
ADU69_RS11010	2255..2413	+	159	WP_145912968.1	hypothetical protein	-
ADU69_RS09990	3134..4069	+	936	WP_062904315.1	replication initiation protein	-
ADU69_RS09995	4360..4863	+	504	WP_046870764.1	DUF536 domain-containing protein	-
ADU69_RS10000	4885..5334	+	450	Protein_7	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11689	11689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T56225 WP_001748110.1 NZ_CP012270:c917-573 [Pediococcus damnosus]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T56225 NZ_CP012270:c917-573 [Pediococcus damnosus]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACGAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.51 Da
• Isoelectric Point: 6.4708

>AT56225 WP_003643337.1 NZ_CP012270:c1174-911 [Pediococcus damnosus]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT56225 NZ_CP012270:c1174-911 [Pediococcus damnosus]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAAGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A6L4ZZT2