Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-vapB/MazF(toxin)
Location 457113..457934 Replicon chromosome
Accession NZ_CP012269
Organism Pediococcus damnosus strain TMW 2.1532

Toxin (Protein)


Gene name pemK Uniprot ID A0A0R2HKP8
Locus tag ADU69_RS02265 Protein ID WP_046871611.1
Coordinates 457563..457934 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0R2HM84
Locus tag ADU69_RS02260 Protein ID WP_046871612.1
Coordinates 457113..457478 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADU69_RS02235 452329..453291 + 963 WP_046871617.1 L-lactate dehydrogenase -
ADU69_RS02240 453475..454866 + 1392 WP_062903867.1 L,D-transpeptidase/peptidoglycan binding protein -
ADU69_RS02245 454999..455646 + 648 WP_046871615.1 CBS domain-containing protein -
ADU69_RS02250 456024..456533 - 510 WP_046871614.1 QueT transporter family protein -
ADU69_RS02255 456628..457116 + 489 WP_046871613.1 GNAT family N-acetyltransferase -
ADU69_RS02260 457113..457478 - 366 WP_046871612.1 DUF488 family protein Antitoxin
ADU69_RS02265 457563..457934 - 372 WP_046871611.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ADU69_RS02270 457953..458225 - 273 WP_141306217.1 hypothetical protein -
ADU69_RS02275 458303..459430 - 1128 WP_062903868.1 alanine racemase -
ADU69_RS02280 459437..459796 - 360 WP_046871608.1 holo-ACP synthase -
ADU69_RS02285 460089..461651 - 1563 WP_046871607.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-113)

Antitoxin

Protein of unknown function DUF488

(4-115)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13241.28 Da        Isoelectric Point: 8.5686

>T56222 WP_046871611.1 NZ_CP012269:c457934-457563 [Pediococcus damnosus]
MSEANVKRGDIFYADLSPVVGSEQGGLRPVLIVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAAETGVERNSVILL
EQIRTIDKQRLKDQVGQLAASKMLEVDTALKVSVGLAENVQHT

Download         Length: 372 bp

>T56222 NZ_CP012269:c457934-457563 [Pediococcus damnosus]
ATGAGTGAAGCCAACGTTAAACGTGGCGACATTTTTTATGCTGACTTGTCTCCTGTAGTTGGGTCTGAACAGGGTGGTTT
GCGCCCTGTTTTAATTGTCCAAAACAATATTGGTAACCACTATAGTCCTACTGTGATTGTGGCAGCTATTACTGCTAAAA
TACAAAAACCAAAAATGCCAACCCACGTTGGAATTACAGCTGCAGAGACGGGTGTTGAACGAAATTCGGTTATTTTGCTA
GAACAAATTCGAACAATTGATAAACAACGACTTAAAGATCAGGTCGGACAATTGGCTGCTAGCAAAATGCTTGAGGTAGA
TACAGCCTTAAAAGTGAGTGTCGGCCTGGCTGAGAATGTTCAACATACATAG

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 14344.42 Da        Isoelectric Point: 7.2021

>AT56222 WP_046871612.1 NZ_CP012269:c457478-457113 [Pediococcus damnosus]
MEIKLERIYTKPADLSGYRVLVDRLWPRGISKVNAQLDWWAKEIGPSTELRKWFDHDPKKFEEFSKKYETELNTAEFSTE
FMTQIKKQLQTQDVIFLYGAKDEKHNQAVILKTYVEAKLAD

Download         Length: 366 bp

>AT56222 NZ_CP012269:c457478-457113 [Pediococcus damnosus]
ATGGAAATTAAGTTGGAACGTATTTATACAAAGCCCGCAGATTTATCGGGATATCGTGTCTTGGTTGACCGATTATGGCC
ACGGGGGATTTCTAAGGTTAACGCTCAGTTAGATTGGTGGGCAAAAGAAATAGGACCAAGTACCGAACTAAGAAAGTGGT
TCGATCATGATCCTAAAAAGTTTGAGGAATTTTCCAAAAAGTATGAAACAGAACTTAACACAGCAGAATTTAGTACGGAA
TTTATGACACAGATTAAAAAGCAACTGCAGACCCAAGACGTAATTTTTTTGTACGGGGCTAAAGACGAAAAACATAACCA
AGCAGTTATTTTGAAAACGTACGTTGAAGCTAAACTTGCAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2HKP8

Antitoxin

Source ID Structure
AlphaFold DB A0A0R2HM84

References