Detailed information of TA system TA056221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4324413..4325002
• Replicon: chromosome
• Accession: NZ_CP012268
• Organism: Cronobacter muytjensii ATCC 51329

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: AFK63_RS19775
• Protein ID: WP_038866959.1
• Coordinates: 4324634..4325002 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: K8BAC3
• Locus tag: AFK63_RS19770
• Protein ID: WP_007730583.1
• Coordinates: 4324413..4324634 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK63_RS19740	4319463..4319855	-	393	WP_038866997.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AFK63_RS19745	4320276..4320533	+	258	WP_038866948.1	amino acid-binding protein	-
AFK63_RS19750	4320598..4321524	+	927	WP_038866950.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AFK63_RS19755	4321521..4322801	+	1281	WP_038866952.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
AFK63_RS19760	4322798..4323565	+	768	WP_038866954.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AFK63_RS19765	4323567..4324280	+	714	WP_038866956.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
AFK63_RS19770	4324413..4324634	+	222	WP_007730583.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AFK63_RS19775	4324634..4325002	+	369	WP_038866959.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AFK63_RS19780	4325173..4326492	+	1320	WP_038866961.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
AFK63_RS19785	4326709..4327596	+	888	WP_038866963.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
AFK63_RS19790	4327593..4328438	+	846	WP_038866965.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
AFK63_RS19795	4328440..4329513	+	1074	WP_038866967.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4310554..4330250	19696

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13519.80 Da
• Isoelectric Point: 6.7259

>T56221 WP_038866959.1 NZ_CP012268:4324634-4325002 [Cronobacter muytjensii ATCC 51329]
MTIHFISAEEIVRFHDRLLQVTPGVTGMPDPGRAEALMYRVLNKYEYEGVDDIWLLAAMHLLAIARGQIFNDGNKRTALF
ITLLLLKRNGIALAANPAFVEMTVNAAAGRLTLEEIAAQLRA

Nucleotide

• Length: 369 bp

>T56221 NZ_CP012268:4324634-4325002 [Cronobacter muytjensii ATCC 51329]
ATGACGATTCACTTTATCTCAGCGGAGGAGATAGTTCGCTTCCATGACCGCTTATTGCAGGTGACGCCCGGCGTCACAGG
GATGCCCGATCCGGGGCGGGCCGAAGCGTTAATGTATCGCGTGCTTAATAAATATGAGTACGAAGGCGTTGACGATATCT
GGCTGCTGGCGGCGATGCATTTACTGGCGATTGCGCGAGGCCAAATTTTCAACGACGGTAATAAGCGCACGGCGCTTTTC
ATTACGCTGTTGCTTCTTAAGCGAAACGGTATCGCGCTTGCGGCTAATCCGGCTTTTGTTGAGATGACCGTCAACGCGGC
GGCAGGGCGTCTGACCCTGGAAGAGATAGCTGCCCAGCTTCGCGCTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8168.20 Da
• Isoelectric Point: 5.6746

>AT56221 WP_007730583.1 NZ_CP012268:4324413-4324634 [Cronobacter muytjensii ATCC 51329]
MRTMNYSEARQQLASALESATTGTPVTITRRGHKPAVIISAEEFERYQAAKLDAEFAEMMAVHGNEIRELADR

Nucleotide

• Length: 222 bp

>AT56221 NZ_CP012268:4324413-4324634 [Cronobacter muytjensii ATCC 51329]
ATGCGTACGATGAACTACAGCGAAGCGCGCCAGCAGCTCGCTTCCGCCCTGGAATCCGCCACCACCGGAACGCCAGTGAC
CATTACGCGCCGCGGCCATAAACCGGCCGTTATCATCAGCGCCGAAGAGTTTGAACGTTACCAGGCGGCCAAACTGGATG
CCGAATTCGCGGAGATGATGGCCGTGCACGGTAATGAAATCAGGGAGCTTGCGGACAGGTAA

Structures

Antitoxin

• AlphaFold DB: K8BAC3