Detailed information of TA system TA056209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 104260..105866
• Replicon: plasmid pCDU1
• Accession: NZ_CP012267
• Organism: Cronobacter dublinensis subsp. dublinensis LMG 23823

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: K8BBE1
• Locus tag: AFK67_RS20955
• Protein ID: WP_007733218.1
• Coordinates: 104260..105579 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: AFK67_RS20960
• Protein ID: WP_007669594.1
• Coordinates: 105582..105866 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK67_RS20945	99642..102173	-	2532	WP_038885052.1	malto-oligosyltrehalose synthase	-
AFK67_RS20950	102170..103954	-	1785	WP_007733217.1	malto-oligosyltrehalose trehalohydrolase	-
AFK67_RS20955	104260..105579	-	1320	WP_007733218.1	type II toxin-antitoxin system HipA family toxin	Toxin
AFK67_RS20960	105582..105866	-	285	WP_007669594.1	helix-turn-helix domain-containing protein	Antitoxin
AFK67_RS20965	105989..106879	-	891	WP_007733221.1	siderophore-interacting protein	-
AFK67_RS20970	106876..108087	-	1212	WP_007669601.1	MFS transporter	-
AFK67_RS20975	108223..109971	+	1749	WP_007669604.1	citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / mgtB / iucA / iucB / iucC / iutA	1..197338	197338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49525.68 Da
• Isoelectric Point: 7.7475

>T56209 WP_007733218.1 NZ_CP012267:c105579-104260 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MRRTPRLAIWMNGIPVGYWDKHLGDDRLEYLPEWTEDAQGRPLSLSLPFTPGNQPYRGTRVRDYFDNLLPDSEGIRRRLA
MRYQAHSLDPFDLLAELGRDCVGAIQLLRADEQPESLFAIRARPLSTVDIAAMLRHTAQAFPGQQGEEETLRLSIAGAQE
KTALLWHNGQWCLPEGNTPTTHIFKLPLGLVGNMQADMRTSIENEWLCSQLLAAYDIPVARTQMAQFEEQKVLIVERFDR
RLSRDKQWWLRLPQEDMCQALGVSPLRKYQSDGGPGIGDIMTILSRSEQARADRATFFRAQIVFWILAATDGHAKNFSIA
HLPGNRYHLTPLYDVLSAWPVIGPGNNQIAWQKCKLAMAVRGSSNYYHLSQIRRRHWLRQGELAGLSHAQVTAMVDALIA
ATPDVIDRVAAALPAGFPLALAEQIFEGMRQQCARLANA

Nucleotide

• Length: 1320 bp

>T56209 NZ_CP012267:c105579-104260 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGCGCCGCACGCCGCGTTTAGCTATCTGGATGAACGGGATCCCGGTCGGCTACTGGGATAAACACCTCGGGGACGATCG
GCTGGAATACCTGCCGGAATGGACTGAGGACGCGCAGGGCCGACCGCTGTCTTTGTCGCTGCCGTTCACCCCGGGCAACC
AGCCGTATCGCGGCACGCGGGTGCGGGATTACTTCGATAACCTGTTGCCGGACAGCGAAGGCATCCGTCGCCGCCTGGCG
ATGCGTTACCAGGCGCACAGCCTCGACCCGTTCGATTTGCTGGCGGAACTGGGACGCGACTGCGTTGGCGCCATCCAGCT
GCTGCGCGCTGATGAACAGCCGGAAAGCCTGTTTGCCATTCGCGCTCGCCCGCTCTCCACAGTGGATATTGCCGCGATGC
TGCGCCATACCGCCCAGGCGTTTCCCGGCCAGCAGGGTGAAGAGGAGACGCTGCGGCTCTCTATCGCAGGCGCACAGGAG
AAAACCGCGCTGCTGTGGCACAACGGTCAGTGGTGTCTGCCGGAGGGCAACACGCCCACAACGCATATCTTCAAACTTCC
GCTGGGACTGGTGGGCAATATGCAGGCGGATATGCGCACGTCGATTGAAAACGAATGGCTCTGTTCGCAGTTACTGGCCG
CGTATGACATTCCCGTCGCACGTACGCAGATGGCGCAGTTTGAAGAGCAAAAAGTGTTGATCGTCGAGCGCTTCGACCGC
CGGTTATCGCGTGATAAACAGTGGTGGCTGCGCTTGCCGCAGGAGGATATGTGCCAGGCGCTCGGCGTGTCGCCGCTGCG
AAAATACCAGTCTGATGGCGGGCCGGGTATCGGCGATATCATGACTATCCTGAGTCGTTCAGAGCAGGCGCGGGCCGATC
GTGCCACTTTCTTCCGCGCGCAGATCGTTTTCTGGATCCTCGCCGCAACAGACGGCCACGCCAAGAACTTCAGCATCGCG
CATCTGCCCGGCAATCGTTATCACCTGACGCCGCTTTATGATGTGTTGTCCGCCTGGCCGGTGATTGGGCCGGGGAATAA
CCAGATTGCATGGCAGAAATGTAAGCTCGCGATGGCGGTGCGGGGCAGCAGCAATTATTACCATCTGAGCCAGATCCGCC
GACGCCACTGGCTGCGCCAGGGCGAGCTTGCGGGGCTGTCGCACGCGCAGGTCACCGCGATGGTAGACGCGCTTATCGCC
GCGACACCCGATGTTATCGACCGCGTGGCCGCTGCGCTTCCGGCTGGCTTCCCCTTAGCGCTGGCGGAGCAGATTTTTGA
GGGCATGCGCCAGCAGTGCGCGCGTCTGGCGAACGCCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10570.06 Da
• Isoelectric Point: 9.8278

>AT56209 WP_007669594.1 NZ_CP012267:c105866-105582 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MTTVYPLKTLSQLRPLLVGFRKNQGLTQREVSERLGVTQQTYARLEANPASASIERLFRVFNVLGIEMTLSEMQKDPSSD
IAASDTSPARREAW

Nucleotide

• Length: 285 bp

>AT56209 NZ_CP012267:c105866-105582 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGACTACCGTCTATCCACTGAAAACGCTCAGCCAGCTGCGCCCGCTGCTGGTGGGCTTTCGCAAAAACCAGGGACTGAC
GCAACGCGAAGTGTCCGAACGACTTGGGGTTACGCAGCAAACGTATGCCCGGCTTGAGGCCAACCCTGCCTCGGCAAGCA
TTGAACGTTTGTTCCGCGTATTTAACGTGCTCGGTATTGAGATGACGCTTAGCGAAATGCAGAAGGATCCCTCCTCTGAT
ATCGCTGCAAGTGACACCTCGCCCGCCCGTCGTGAGGCGTGGTGA

Structures

Toxin

• AlphaFold DB: K8BBE1