Detailed information of TA system TA056195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1159749..1160368
• Replicon: chromosome
• Accession: NZ_CP012266
• Organism: Cronobacter dublinensis subsp. dublinensis LMG 23823

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: K8B192
• Locus tag: AFK67_RS05315
• Protein ID: WP_007713751.1
• Coordinates: 1159749..1159967 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: K8AHS7
• Locus tag: AFK67_RS05320
• Protein ID: WP_007713750.1
• Coordinates: 1159994..1160368 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK67_RS05295	1155872..1157773	+	1902	WP_007713756.1	methyl-accepting chemotaxis protein	-
AFK67_RS05300	1157915..1158175	+	261	WP_007713755.1	type B 50S ribosomal protein L31	-
AFK67_RS21575	1158179..1158319	+	141	WP_007713754.1	type B 50S ribosomal protein L36	-
AFK67_RS05305	1158349..1158858	-	510	WP_007713753.1	YlaC family protein	-
AFK67_RS05310	1158978..1159529	-	552	WP_038884059.1	maltose O-acetyltransferase	-
AFK67_RS05315	1159749..1159967	-	219	WP_007713751.1	hemolysin expression modulator Hha	Toxin
AFK67_RS05320	1159994..1160368	-	375	WP_007713750.1	Hha toxicity modulator TomB	Antitoxin
AFK67_RS05325	1160887..1164036	-	3150	WP_007713749.1	multidrug efflux RND transporter permease subunit	-
AFK67_RS05330	1164058..1165260	-	1203	WP_007713748.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8624.06 Da
• Isoelectric Point: 9.4828

>T56195 WP_007713751.1 NZ_CP012266:c1159967-1159749 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELPDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T56195 NZ_CP012266:c1159967-1159749 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGCGACGCTGTCAGACAATTGATACGCTTGAGCGTGT
GATTGAAAAAAATAAATATGAATTGCCCGACAACGAACTGGCGGTATTTTATTCAGCAGCGGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTACGATAAAATCCCAACGTCCGTGTGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14417.15 Da
• Isoelectric Point: 4.7269

>AT56195 WP_007713750.1 NZ_CP012266:c1160368-1159994 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MDEYSPKRHDMAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFALNYKIKHEEDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFVNASRENPVSLSF

Nucleotide

• Length: 375 bp

>AT56195 NZ_CP012266:c1160368-1159994 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGGACGAATACTCACCAAAACGGCATGATATGGCGCAGCTTAAATTTCTGTGTGAAAGCCTGTATCATGATTGCCTCGC
CACGCTCGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACATCCGCCGTTAACCTGCAACTGAACGATCTGATTGAGC
ACATTGCCAGCTTCGCGCTTAATTATAAAATTAAGCATGAAGAGGATGCCGCTCTCATTGAACAATTGGATGAATATCTG
GACGATACCTTTATGCTTTTCAGCAATTACGGTATCAATGCGCAAGATTTGCAAAAATGGCGCAAGTCCGGAAACAGACT
TTTCCGGTGTTTCGTAAACGCCAGCCGGGAGAATCCTGTGAGCTTGTCTTTTTAA

Structures

Toxin

• AlphaFold DB: K8B192

Antitoxin

• AlphaFold DB: K8AHS7