Detailed information of TA system TA056194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 198747..199552
• Replicon: chromosome
• Accession: NZ_CP012266
• Organism: Cronobacter dublinensis subsp. dublinensis LMG 23823

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: K8AQB1
• Locus tag: AFK67_RS00955
• Protein ID: WP_007729191.1
• Coordinates: 199025..199552 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: K8AR51
• Locus tag: AFK67_RS00950
• Protein ID: WP_007729189.1
• Coordinates: 198747..199028 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK67_RS00930	193896..194573	+	678	WP_007729166.1	6-N-hydroxylaminopurine resistance protein	-
AFK67_RS00935	194677..195882	-	1206	WP_007729169.1	FMN-dependent L-lactate dehydrogenase LldD	-
AFK67_RS00940	195882..196667	-	786	WP_007729172.1	transcriptional regulator LldR	-
AFK67_RS00945	196664..198319	-	1656	WP_007729175.1	L-lactate permease	-
AFK67_RS00950	198747..199028	+	282	WP_007729189.1	DUF1778 domain-containing protein	Antitoxin
AFK67_RS00955	199025..199552	+	528	WP_007729191.1	GNAT family N-acetyltransferase	Toxin
AFK67_RS00960	199592..199954	-	363	WP_007729196.1	YibL family ribosome-associated protein	-
AFK67_RS00965	200085..201599	-	1515	WP_032967552.1	glycerol kinase GlpK	-
AFK67_RS00970	201621..202469	-	849	WP_007729203.1	aquaporin family protein	-
AFK67_RS00975	202904..203143	+	240	WP_007667757.1	septal ring assembly protein ZapB	-
AFK67_RS00980	203194..203679	-	486	WP_007729210.1	ribonuclease E activity regulator RraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-84)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19492.30 Da
• Isoelectric Point: 9.0690

>T56194 WP_007729191.1 NZ_CP012266:199025-199552 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MTLKRGVFPYTPEHGWKTASFDCGCDTLNGFLRQRLAVQQQRNVLRAYVVLTNEPEPAVAGYYTLSGTSFERSLFGDRKR
QIPYDRAPCVLLGRLAVDKTHAGKAIGSMLVADAARRVYDVARAVGVYAMFVEAKDAYAAQFYQQLGFLQAESTDGRQCL
FFPIRALDALFTTTS

Nucleotide

• Length: 528 bp

>T56194 NZ_CP012266:199025-199552 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGACGCTGAAGCGAGGCGTTTTTCCTTACACACCGGAGCACGGCTGGAAGACGGCGTCGTTCGACTGCGGCTGTGACAC
ACTGAATGGTTTTCTTCGCCAGCGCCTTGCAGTGCAGCAACAGCGCAATGTTCTGCGTGCGTATGTCGTGTTAACCAACG
AGCCGGAGCCTGCGGTCGCTGGCTACTACACGCTGTCGGGCACCTCTTTCGAGAGGTCGCTGTTTGGCGATCGGAAGCGG
CAGATCCCTTACGATAGGGCGCCATGCGTGTTACTGGGCAGGCTGGCGGTAGACAAAACACATGCGGGAAAGGCCATCGG
GTCGATGCTGGTGGCGGATGCCGCGAGACGCGTTTATGACGTCGCTCGTGCGGTAGGCGTCTATGCGATGTTTGTGGAAG
CGAAAGATGCGTACGCCGCACAGTTTTATCAGCAGCTCGGATTCCTGCAAGCAGAAAGTACCGACGGGCGACAGTGCCTG
TTTTTTCCGATCAGAGCACTCGACGCGTTATTCACTACAACATCATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10459.74 Da
• Isoelectric Point: 5.6565

>AT56194 WP_007729189.1 NZ_CP012266:198747-199028 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MPEPSKKDRLECRLSTEDKAAIEAAASLSNTSVSQFIVDAARARARVLLEEHHRIRLTEQGWHAVMAALDNPPASNEKLK
RAFESLDNGEFWE

Nucleotide

• Length: 282 bp

>AT56194 NZ_CP012266:198747-199028 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGCCTGAGCCGTCGAAAAAAGACCGTCTGGAGTGTCGTCTCTCCACGGAGGACAAAGCTGCTATTGAAGCGGCTGCCAG
CCTCTCAAACACCAGCGTCTCGCAATTTATTGTCGATGCCGCTCGCGCTCGCGCCCGAGTCTTGCTTGAAGAACATCACC
GTATTCGGTTAACGGAGCAGGGCTGGCATGCCGTTATGGCGGCGCTGGATAACCCACCCGCGTCAAACGAGAAACTCAAG
CGTGCGTTTGAAAGCCTGGATAACGGTGAATTCTGGGAATGA

Structures

Toxin

• AlphaFold DB: K8AQB1

Antitoxin

• AlphaFold DB: K8AR51