Detailed information of TA system TA056181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1154167..1154786
• Replicon: chromosome
• Accession: NZ_CP012264
• Organism: Cronobacter condimenti 1330 strain LMG 26250

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: K8AAX3
• Locus tag: AFK62_RS05300
• Protein ID: WP_007673679.1
• Coordinates: 1154167..1154385 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: K8A0P1
• Locus tag: AFK62_RS05305
• Protein ID: WP_007673676.1
• Coordinates: 1154412..1154786 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK62_RS05275	1150286..1152187	+	1902	WP_007673694.1	methyl-accepting chemotaxis protein	-
AFK62_RS05280	1152330..1152590	+	261	WP_007673691.1	type B 50S ribosomal protein L31	-
AFK62_RS05285	1152594..1152734	+	141	WP_007673688.1	type B 50S ribosomal protein L36	-
AFK62_RS05290	1152767..1153276	-	510	WP_007673685.1	YlaC family protein	-
AFK62_RS05295	1153396..1153947	-	552	WP_007673683.1	maltose O-acetyltransferase	-
AFK62_RS05300	1154167..1154385	-	219	WP_007673679.1	hemolysin expression modulator Hha	Toxin
AFK62_RS05305	1154412..1154786	-	375	WP_007673676.1	Hha toxicity modulator TomB	Antitoxin
AFK62_RS05310	1155251..1158400	-	3150	WP_007673672.1	multidrug efflux RND transporter permease subunit	-
AFK62_RS05315	1158422..1159627	-	1206	WP_032984453.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.10 Da
• Isoelectric Point: 9.4828

>T56181 WP_007673679.1 NZ_CP012264:c1154385-1154167 [Cronobacter condimenti 1330]
MTTKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T56181 NZ_CP012264:c1154385-1154167 [Cronobacter condimenti 1330]
ATGACGACCAAACCATTAACCAAAATTGATTATTTAATGCGACTGAGACGCTGTCAGACAATTGATACGCTCGAGCGCGT
TATTGAAAAAAATAAATATGAATTATCTGACAATGAACTGGCGGTATTTTATTCAGCAGCAGATCATCGCCTGGCTGAAC
TCACGATGAATAAGCTCTACGATAAAATCCCAACGTCTGTGTGGAAATTCATCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14387.06 Da
• Isoelectric Point: 4.6025

>AT56181 WP_007673676.1 NZ_CP012264:c1154786-1154412 [Cronobacter condimenti 1330]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFALNYKIKHEEDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFDNASKENPVSLSF

Nucleotide

• Length: 375 bp

>AT56181 NZ_CP012264:c1154786-1154412 [Cronobacter condimenti 1330]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTTTGCGAAAGCCTGTATCATGATTGCCTCGC
CACGCTCGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACATCTGCCGTTAACCTGCAGCTGAACGATCTGATTGAGC
ATATAGCCAGCTTCGCGCTTAATTATAAAATTAAGCATGAAGAAGATGCTGCTCTCATTGAACAATTGGATGAATATCTG
GACGATACCTTTATGCTTTTCAGCAATTACGGGATCAATGCGCAAGATTTGCAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTTCGGTGTTTCGACAACGCCAGCAAAGAAAATCCTGTGAGCTTGTCTTTTTAA

Structures

Toxin

• AlphaFold DB: K8AAX3

Antitoxin

• AlphaFold DB: K8A0P1