Detailed information of TA system TA056180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 55939..57542
• Replicon: plasmid pCUNV1
• Accession: NZ_CP012258
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A376KQ45
• Locus tag: AFK65_RS20155
• Protein ID: WP_038858818.1
• Coordinates: 56223..57542 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: AFK65_RS20150
• Protein ID: WP_007706016.1
• Coordinates: 55939..56220 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS20135	51822..53573	-	1752	WP_038858814.1	aerobactin synthase IucA	-
AFK65_RS20140	53709..54920	+	1212	WP_038858816.1	MFS transporter	-
AFK65_RS20145	54917..55804	+	888	WP_038858817.1	siderophore-interacting protein	-
AFK65_RS20150	55939..56220	+	282	WP_007706016.1	helix-turn-helix domain-containing protein	Antitoxin
AFK65_RS20155	56223..57542	+	1320	WP_038858818.1	type II toxin-antitoxin system HipA family toxin	Toxin
AFK65_RS20160	57846..59630	+	1785	WP_038858819.1	malto-oligosyltrehalose trehalohydrolase	-
AFK65_RS20165	59627..62158	+	2532	WP_007706011.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / iutA / iucC / iucB / iucA / mgtB	1..129777	129777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (153-405)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49060.08 Da
• Isoelectric Point: 7.2481

>T56180 WP_038858818.1 NZ_CP012258:56223-57542 [Cronobacter universalis NCTC 9529]
MRRTSRLALWMNGLPVGYWDKHNGEDRLTYLPEWIADPQGRPLSLSLPFTPGNQSYRGARVRDYFDNLLPDSENIRRRLA
MRYRAQSLEPFDLLAELGRDCVGAIQLLPAGEAPEGLFTIRARPLTTVDIAAMLRHTAETFPGQHGGEEELRLSIAGAQE
KTALLWHEGQWCLPEGNTPTTHIFKLPLGLVGNMQADMRTSIENEWLCAQLLEAWDIPVARTQMAQFDEQKVLIVERFDR
RLSADGQWWLRLPQEDMCQALGVSPLRKYQADGGPGIGDIMTILSRSENAQADRAHFFRAQIIFWILAATDGHAKNFSIA
HLPGNRYHLTPLYDVLSAWPVIGPGNNQIAWQKCKLAMAVRGGSNYYHLSQIRRRHWLRQGELAGLSAAQVNDMIDALTA
SAPAAIARVADALPAGFPATLADVIFNGMRQQCARLAAP

Nucleotide

• Length: 1320 bp

>T56180 NZ_CP012258:56223-57542 [Cronobacter universalis NCTC 9529]
ATGCGCCGCACGTCGCGTTTAGCGCTCTGGATGAACGGTCTCCCGGTTGGTTACTGGGATAAACACAACGGCGAAGACCG
GCTGACTTATCTGCCGGAATGGATTGCCGATCCGCAGGGCAGACCGCTGTCGCTGTCGCTGCCGTTCACGCCCGGCAATC
AGTCATACCGCGGCGCGCGGGTGCGCGATTACTTCGACAACCTGCTGCCGGACAGTGAAAACATCCGGCGGCGGCTGGCG
ATGCGCTACCGGGCGCAGAGCCTGGAGCCGTTCGATCTGCTGGCGGAGCTGGGACGCGACTGCGTCGGCGCCATTCAGCT
GCTGCCTGCCGGTGAAGCGCCGGAAGGGCTCTTTACCATTCGCGCCCGGCCACTCACCACAGTGGATATTGCCGCGATGC
TGCGCCACACCGCAGAGACCTTTCCCGGCCAGCATGGCGGGGAAGAGGAGCTGCGGCTCTCCATTGCGGGCGCGCAGGAG
AAAACCGCGCTGCTGTGGCACGAAGGCCAGTGGTGCCTGCCGGAGGGCAATACGCCCACCACGCATATTTTCAAACTGCC
GCTGGGGCTGGTGGGCAATATGCAGGCGGACATGCGCACCTCGATTGAAAACGAATGGCTCTGCGCGCAGCTGCTGGAAG
CCTGGGATATTCCGGTCGCGCGCACGCAGATGGCGCAGTTCGATGAGCAAAAAGTGCTGATTGTCGAACGCTTCGACCGG
CGTCTTTCGGCCGATGGGCAGTGGTGGCTGCGTCTGCCGCAGGAGGATATGTGCCAGGCGCTCGGCGTCTCGCCGCTGCG
TAAATATCAGGCCGACGGCGGGCCCGGCATCGGCGATATCATGACTATCCTGAGCCGTTCGGAAAACGCGCAGGCGGATC
GCGCGCATTTTTTCCGCGCCCAGATTATCTTCTGGATCCTCGCCGCGACCGACGGCCACGCCAAGAACTTCAGCATCGCG
CACCTGCCCGGCAACCGTTACCACCTGACGCCGCTCTATGACGTCCTTTCCGCCTGGCCGGTTATCGGGCCGGGTAATAA
CCAGATTGCGTGGCAGAAGTGCAAGCTGGCGATGGCGGTGCGCGGCGGCAGTAACTATTACCATCTGAGCCAGATCCGCC
GCCGTCACTGGCTGCGCCAGGGCGAACTCGCCGGGCTGTCGGCGGCGCAGGTTAACGACATGATTGATGCGCTGACAGCC
AGCGCGCCTGCGGCGATCGCACGCGTGGCCGACGCGCTTCCGGCGGGTTTTCCCGCTACTCTGGCCGACGTTATTTTTAA
CGGCATGCGCCAGCAGTGCGCGCGCCTCGCGGCCCCCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10466.94 Da
• Isoelectric Point: 7.7916

>AT56180 WP_007706016.1 NZ_CP012258:55939-56220 [Cronobacter universalis NCTC 9529]
MNTRYPLKTLSQLRPLLVGFRKNQGLTQKEVSARLGVTQQTYARLEASPETASIERLFRVFNVLGIEITLCETQTAEADA
PVCDDSPARREAW

Nucleotide

• Length: 282 bp

>AT56180 NZ_CP012258:55939-56220 [Cronobacter universalis NCTC 9529]
ATGAATACCCGGTATCCACTGAAAACGCTCAGCCAGTTGCGCCCGCTGCTGGTGGGCTTTCGCAAAAACCAGGGGCTGAC
CCAGAAAGAGGTCTCGGCGCGGCTTGGCGTCACCCAGCAAACCTATGCGCGGCTGGAGGCGAGCCCCGAAACGGCCAGCA
TCGAGCGCCTGTTCCGCGTTTTTAACGTCCTCGGCATTGAGATAACGCTGTGCGAAACGCAGACGGCTGAGGCTGACGCG
CCCGTCTGTGACGATTCGCCTGCCCGCCGCGAGGCGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A376KQ45