Detailed information of TA system TA056178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4055213..4055723
• Replicon: chromosome
• Accession: NZ_CP012257
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A376EYF5
• Locus tag: AFK65_RS18745
• Protein ID: WP_007702964.1
• Coordinates: 4055213..4055521 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: AFK65_RS18750
• Protein ID: WP_007702967.1
• Coordinates: 4055523..4055723 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS18725	4050496..4051254	-	759	WP_038858678.1	SDR family oxidoreductase	-
AFK65_RS18730	4051373..4052272	+	900	WP_038858676.1	LysR family transcriptional regulator	-
AFK65_RS18735	4052325..4053359	+	1035	WP_007702956.1	inner membrane protein YhjD	-
AFK65_RS18740	4053633..4054961	+	1329	WP_038858674.1	MHS family MFS transporter	-
AFK65_RS18745	4055213..4055521	-	309	WP_007702964.1	CcdB family protein	Toxin
AFK65_RS18750	4055523..4055723	-	201	WP_007702967.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AFK65_RS18755	4055880..4057937	-	2058	WP_038858672.1	AsmA family protein	-
AFK65_RS18760	4058002..4058775	-	774	WP_007702972.1	cyclic-guanylate-specific phosphodiesterase	-
AFK65_RS18765	4059009..4059941	+	933	WP_007702976.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (1-66)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11672.63 Da
• Isoelectric Point: 8.8697

>T56178 WP_007702964.1 NZ_CP012257:c4055521-4055213 [Cronobacter universalis NCTC 9529]
MQYQIYRNTSANKKYPYLIDIQSDLVDVLTTRIVIPLVPRFMAQKPLPERMCPIVNVDGDKYIVMTHEMASVGKSVLGQH
TASALPWRQQIKNAVDFVFDGF

Nucleotide

• Length: 309 bp

>T56178 NZ_CP012257:c4055521-4055213 [Cronobacter universalis NCTC 9529]
ATGCAATACCAAATCTATCGCAATACCAGCGCCAATAAAAAGTATCCGTATCTGATAGATATACAGAGCGATCTGGTTGA
TGTGCTGACCACACGTATCGTTATCCCGCTCGTGCCACGTTTTATGGCGCAAAAGCCGTTGCCTGAGCGAATGTGCCCGA
TTGTTAATGTGGACGGTGATAAGTACATCGTGATGACGCATGAAATGGCGAGCGTAGGTAAAAGCGTACTCGGCCAGCAT
ACCGCCAGCGCGTTGCCCTGGCGCCAGCAGATTAAAAACGCCGTCGATTTTGTGTTTGACGGATTTTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7706.51 Da
• Isoelectric Point: 4.6363

>AT56178 WP_007702967.1 NZ_CP012257:c4055723-4055523 [Cronobacter universalis NCTC 9529]
VTIDESLYQRARAHGINLSQRLSLVLEDDLRELEREQWKDDNREGLLELNGIAGECGHFADEHRKF

Nucleotide

• Length: 201 bp

>AT56178 NZ_CP012257:c4055723-4055523 [Cronobacter universalis NCTC 9529]
GTGACCATAGATGAATCGTTATATCAGCGCGCCAGGGCACATGGTATTAATCTCTCCCAGCGGCTAAGCCTGGTGCTGGA
AGACGATTTGCGTGAGCTTGAGCGTGAGCAATGGAAAGATGACAACCGCGAAGGATTGCTGGAGTTAAATGGGATCGCCG
GGGAGTGCGGTCATTTTGCGGATGAACACCGGAAGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A376EYF5