Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 3848202..3848787 Replicon chromosome
Accession NZ_CP012257
Organism Cronobacter universalis NCTC 9529

Toxin (Protein)


Gene name PumA Uniprot ID A0A376EZK7
Locus tag AFK65_RS17710 Protein ID WP_032804980.1
Coordinates 3848202..3848507 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A376EX34
Locus tag AFK65_RS17715 Protein ID WP_007702390.1
Coordinates 3848512..3848787 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK65_RS17685 3844234..3845826 + 1593 WP_007702375.1 RNA repair transcriptional activator RtcR -
AFK65_RS17690 3845989..3846921 + 933 WP_007702376.1 ketopantoate/pantoate/pantothenate transporter PanS -
AFK65_RS17695 3847012..3847284 + 273 WP_007702377.1 DUF3811 domain-containing protein -
AFK65_RS17700 3847315..3847638 - 324 WP_007702380.1 helix-turn-helix domain-containing protein -
AFK65_RS17705 3847635..3847985 - 351 WP_007702383.1 type II toxin-antitoxin system RelE/ParE family toxin -
AFK65_RS17710 3848202..3848507 + 306 WP_032804980.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AFK65_RS17715 3848512..3848787 + 276 WP_007702390.1 putative addiction module antidote protein Antitoxin
AFK65_RS17720 3848804..3850435 - 1632 WP_038856148.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3840594..3848787 8193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-93)

Antitoxin

Uncharacterized protein HI1420

(2-89)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11574.55 Da        Isoelectric Point: 10.3395

>T56175 WP_032804980.1 NZ_CP012257:3848202-3848507 [Cronobacter universalis NCTC 9529]
MKHIFQTEVFRRWQASLKDQRVKTMIAARLFRLANGLPGDVKPVGAGISELRIHYGAGYRIYFKQHGDVIVILLCGGDKH
TQDKDILLAKMLADLPENQWR

Download         Length: 306 bp

>T56175 NZ_CP012257:3848202-3848507 [Cronobacter universalis NCTC 9529]
ATGAAGCATATTTTTCAAACTGAAGTATTCCGCCGTTGGCAAGCCAGTCTGAAAGACCAGCGGGTAAAAACGATGATTGC
CGCGCGGCTTTTTCGGCTGGCTAATGGTTTGCCTGGTGATGTTAAGCCCGTCGGCGCAGGCATCAGCGAGCTGCGTATTC
ACTACGGCGCGGGATACCGGATTTATTTCAAACAACACGGCGACGTGATTGTCATTTTGCTTTGTGGTGGTGATAAGCAC
ACGCAGGATAAAGACATTCTTCTTGCGAAAATGCTGGCTGACCTGCCAGAGAACCAGTGGAGATAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9655.14 Da        Isoelectric Point: 6.4609

>AT56175 WP_007702390.1 NZ_CP012257:3848512-3848787 [Cronobacter universalis NCTC 9529]
MKLTEYDPAAALIDDEEIAFFMADALETGDAGHIAKALGIVARAKGMSHIASQTGLSREQLYRSFSDKGNPTLKTTLAVM
KALGLGLTIKS

Download         Length: 276 bp

>AT56175 NZ_CP012257:3848512-3848787 [Cronobacter universalis NCTC 9529]
ATGAAGCTGACCGAATACGATCCCGCTGCGGCGCTGATTGATGATGAGGAGATCGCCTTCTTTATGGCGGACGCGCTCGA
AACTGGCGACGCCGGACATATTGCAAAGGCGCTGGGCATCGTCGCGCGCGCTAAAGGCATGTCGCATATCGCCTCTCAGA
CAGGGTTGTCGCGTGAACAGCTGTACCGTTCTTTCAGTGATAAAGGCAACCCAACGCTTAAAACCACGCTGGCAGTGATG
AAAGCGCTGGGTCTCGGTTTAACGATTAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376EZK7

Antitoxin

Source ID Structure
AlphaFold DB A0A376EX34

References