Detailed information of TA system TA056174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3847315..3847985
• Replicon: chromosome
• Accession: NZ_CP012257
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A376EZ60
• Locus tag: AFK65_RS17705
• Protein ID: WP_007702383.1
• Coordinates: 3847635..3847985 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: AFK65_RS17700
• Protein ID: WP_007702380.1
• Coordinates: 3847315..3847638 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS17680	3842625..3843758	-	1134	WP_007702374.1	slipin family protein	-
AFK65_RS17685	3844234..3845826	+	1593	WP_007702375.1	RNA repair transcriptional activator RtcR	-
AFK65_RS17690	3845989..3846921	+	933	WP_007702376.1	ketopantoate/pantoate/pantothenate transporter PanS	-
AFK65_RS17695	3847012..3847284	+	273	WP_007702377.1	DUF3811 domain-containing protein	-
AFK65_RS17700	3847315..3847638	-	324	WP_007702380.1	helix-turn-helix domain-containing protein	Antitoxin
AFK65_RS17705	3847635..3847985	-	351	WP_007702383.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK65_RS17710	3848202..3848507	+	306	WP_032804980.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AFK65_RS17715	3848512..3848787	+	276	WP_007702390.1	putative addiction module antidote protein	-
AFK65_RS17720	3848804..3850435	-	1632	WP_038856148.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3840594..3848787	8193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13009.08 Da
• Isoelectric Point: 10.0077

>T56174 WP_007702383.1 NZ_CP012257:c3847985-3847635 [Cronobacter universalis NCTC 9529]
MIKPLFWVGQARKDLQALPEDVQDLFGYALYLAQQGRRHPQAKPLKGFGGAGVLEVVEDNQGNTFRAVYTVRLREAIYVL
HVFQKKSSSCIATPRPEMDKIEQRLKAAQCHAGGLK

Nucleotide

• Length: 351 bp

>T56174 NZ_CP012257:c3847985-3847635 [Cronobacter universalis NCTC 9529]
ATGATAAAACCGTTGTTTTGGGTCGGGCAGGCCAGAAAAGATCTTCAGGCGCTGCCGGAGGATGTTCAGGATCTTTTTGG
TTATGCGCTTTACCTGGCGCAGCAGGGCAGACGCCATCCGCAGGCTAAACCCCTCAAAGGATTCGGCGGCGCGGGTGTGC
TTGAGGTGGTGGAGGATAACCAGGGAAATACCTTTAGGGCGGTCTATACCGTGCGCCTGAGAGAGGCGATTTATGTCCTG
CACGTTTTCCAGAAGAAATCCTCTTCCTGTATTGCCACGCCCCGACCGGAAATGGACAAAATTGAACAGCGACTGAAAGC
CGCGCAATGCCACGCCGGAGGGCTGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11808.91 Da
• Isoelectric Point: 7.9747

>AT56174 WP_007702380.1 NZ_CP012257:c3847638-3847315 [Cronobacter universalis NCTC 9529]
MTADIERSNGNVYDDLGLIDAEEMQLKAQLAMAITDLLKARGLTQQEAARLLGMTQPKLSLLLRGQFRGISETKMMACLM
LLGRDISIVVGEAQQARGKMRLIYQPA

Nucleotide

• Length: 324 bp

>AT56174 NZ_CP012257:c3847638-3847315 [Cronobacter universalis NCTC 9529]
ATGACAGCAGACATTGAACGCAGTAACGGAAATGTGTATGACGATCTCGGGCTTATCGATGCCGAAGAGATGCAGCTAAA
AGCGCAGCTCGCGATGGCCATTACCGACCTTCTTAAAGCGCGCGGCTTAACCCAGCAGGAGGCGGCACGTCTTCTGGGCA
TGACCCAGCCAAAACTCTCTTTGTTACTTCGCGGCCAGTTTCGCGGGATCAGCGAAACGAAAATGATGGCCTGCCTGATG
CTCCTCGGGCGAGATATCAGCATCGTCGTGGGCGAGGCACAACAGGCGCGCGGAAAAATGCGCCTGATCTATCAGCCCGC
CTGA

Structures

Toxin

• AlphaFold DB: A0A376EZ60