Detailed information of TA system TA056158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 14..657
• Replicon: plasmid pCS3
• Accession: NZ_CP012256
• Organism: Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AFK64_RS21125
• Protein ID: WP_015387203.1
• Coordinates: 241..657 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: AFK64_RS21120
• Protein ID: WP_015387204.1
• Coordinates: 14..244 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK64_RS21120	14..244	+	231	WP_015387204.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AFK64_RS21125	241..657	+	417	WP_015387203.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFK64_RS21130	1076..1369	+	294	WP_015387202.1	hypothetical protein	-
AFK64_RS21135	1426..1749	+	324	WP_015387201.1	hypothetical protein	-
AFK64_RS21140	1753..2523	-	771	WP_015387200.1	DUF4225 domain-containing protein	-
AFK64_RS21145	2914..3321	+	408	WP_015387199.1	glutathione S-transferase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53383	53383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14924.31 Da
• Isoelectric Point: 7.2449

>T56158 WP_015387203.1 NZ_CP012256:241-657 [Cronobacter sakazakii]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAN

Nucleotide

• Length: 417 bp

>T56158 NZ_CP012256:241-657 [Cronobacter sakazakii]
GTGATCAAGACCTATATGTTGGACACCAACATTTGCTCATTCATTATGCGTGAGCAACCGGAAGCTGTCATCAAACGGCT
GGAACAGGCAGTACTGCGCAACCACCGCATTGTGGTTTCGGCCATTACCTATGCCGAGATGCGTTTCGGTGCGATCGGAA
AGAAGGCATCTCCCCGGCACGGCCTGCTTGTCGAAGCTTTCTGCGCCCGCCTCGATGCAATCCTCGCCTGGGACCGCGCC
GCAGTAGACGCAACCACAGAGATTAAGGCTGCGCTGACTGCGGCCGGGACGCCAATCGGTCCGAACGACACGGCGATTGC
CGGGCATGCTATCGCCGCCGGCGCCGTACTAGTGACGAACAACGTGCGAGAGTTTGAGCGTGTGCCTGGACTGGTTCTGG
AAGACTGGGCTAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.6182

>AT56158 WP_015387204.1 NZ_CP012256:14-244 [Cronobacter sakazakii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFRQLEKADPDFMAERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT56158 NZ_CP012256:14-244 [Cronobacter sakazakii]
ATGAGAACCGTATCGATATTTAAAAACGGCAATAATCGTGCTATCCGTCTGCCCCGAGACCTGGATTTTGAGGGTGTGAG
TGAACTGGAGATCGTCCGGGAAGGTGACAGCATCATTCTTCGTCCCGTTCGCCCAACATGGGGTTCGTTCAGGCAACTCG
AAAAAGCCGATCCGGACTTTATGGCCGAACGTGGGGACGTTGTCAGCGATGAAGGACGATTTGACCTGTGA