Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 88807..90410 Replicon plasmid pCS2
Accession NZ_CP012255
Organism Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag AFK64_RS20990 Protein ID WP_015387275.1
Coordinates 88807..90126 (-) Length 440 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag AFK64_RS20995 Protein ID WP_007854617.1
Coordinates 90129..90410 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK64_RS20980 84189..86720 - 2532 WP_015387273.1 malto-oligosyltrehalose synthase -
AFK64_RS20985 86717..88501 - 1785 WP_015387274.1 malto-oligosyltrehalose trehalohydrolase -
AFK64_RS20990 88807..90126 - 1320 WP_015387275.1 type II toxin-antitoxin system HipA family toxin Toxin
AFK64_RS20995 90129..90410 - 282 WP_007854617.1 helix-turn-helix domain-containing protein Antitoxin
AFK64_RS21000 90541..91425 - 885 WP_015387276.1 siderophore-interacting protein -
AFK64_RS21005 91422..92633 - 1212 WP_015387277.1 MFS transporter -
AFK64_RS21010 92769..94517 + 1749 WP_029463996.1 aerobactin synthase IucA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - vipA/tssB / vipB/tssC / mgtB / iucA / iucB / iucC / iutA / pla 1..117795 117795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 440 a.a.        Molecular weight: 49009.97 Da        Isoelectric Point: 6.8730

>T56157 WP_015387275.1 NZ_CP012255:c90126-88807 [Cronobacter sakazakii]
MRRTSRLALWMNGLPVGYWDKHNGEDRLTYLPEWIADPQGRPLSLSLPFTPGNQSYRGAQVRDYFDNLLPDSENIRRRLA
MRYRAQSLEPFDLLAELGRDCVGAIQLLPAGEAPEGLFTIRARPLSTVDIAAMLRHTAEAFPGQHGGEEELRLSIAGAQE
KTALLWHEGQWCLPEGNTPTTHIFKLPLGLVGNMQADMRTSIENEWLCAQLLAAWDIPVAHTQMAQFEEQKVLIVERFDR
RLSADGQWWLRLPQEDMCQALGVSPLRKYQADGGPGIGDIMTILSRSENAQADRANFFRAQIIFWILAATDGHAKNFSIA
HLPGNRYHLTPLYDVLSAWPVIGPGNNQIAWQKCKLAMAVRGGSNYYHLSQIRRRHWLRQGELAGLSAAQVNDMIDALTA
SAPAVFARVADALPVDFPASLADVIFNGMRQQCARLAAA

Download         Length: 1320 bp

>T56157 NZ_CP012255:c90126-88807 [Cronobacter sakazakii]
ATGCGCCGCACGTCGCGTTTAGCGCTCTGGATGAACGGCCTGCCGGTCGGTTACTGGGATAAACACAACGGCGAAGACCG
GCTGACCTATCTGCCGGAGTGGATTGCCGATCCGCAGGGCAGGCCGCTGTCGCTGTCGCTGCCGTTCACGCCCGGTAATC
AGTCATACCGTGGCGCGCAGGTGCGCGATTACTTCGACAACCTGCTGCCGGACAGCGAAAACATCCGGCGGCGGCTGGCG
ATGCGCTACCGGGCGCAGAGCCTGGAGCCGTTCGATCTGCTGGCGGAGCTGGGACGCGACTGTGTGGGCGCCATCCAGCT
GTTGCCCGCCGGTGAAGCACCGGAAGGGCTTTTTACCATCCGCGCCCGTCCACTCTCCACAGTGGATATTGCCGCGATGC
TGCGCCACACCGCAGAGGCGTTCCCCGGCCAGCATGGCGGCGAAGAGGAGCTGCGCCTCTCCATTGCGGGCGCGCAGGAG
AAAACCGCGCTGCTGTGGCACGAAGGCCAGTGGTGCCTGCCGGAGGGCAACACGCCCACCACGCATATTTTCAAACTGCC
GCTGGGGCTGGTGGGCAATATGCAGGCGGATATGCGCACCTCCATTGAAAACGAATGGCTCTGTGCGCAACTGCTGGCGG
CCTGGGATATTCCGGTCGCGCACACGCAGATGGCGCAGTTCGAAGAGCAAAAAGTGCTGATTGTCGAACGCTTCGACCGG
CGTCTTTCGGCCGATGGCCAGTGGTGGCTGCGTCTGCCGCAGGAGGATATGTGCCAGGCGCTGGGCGTCTCGCCGCTGCG
TAAATATCAGGCCGACGGCGGGCCGGGCATCGGGGATATCATGACCATCCTGAGCCGTTCAGAAAACGCGCAGGCGGATC
GCGCGAATTTCTTCCGCGCGCAGATTATTTTCTGGATCCTCGCCGCGACCGACGGCCACGCCAAGAATTTCAGCATTGCG
CATCTGCCCGGCAACCGCTACCACCTGACGCCGCTTTACGACGTGCTCTCCGCCTGGCCGGTCATCGGGCCGGGTAATAA
CCAGATTGCGTGGCAGAAGTGCAAGCTGGCGATGGCGGTGCGCGGCGGCAGTAACTATTACCACCTGAGCCAGATCCGCC
GCCGTCACTGGCTGCGCCAGGGCGAGCTTGCCGGGCTGTCGGCGGCGCAGGTCAACGACATGATTGATGCGCTGACAGCC
AGCGCGCCTGCGGTGTTCGCGCGCGTGGCCGACGCGCTCCCGGTGGATTTCCCCGCCTCTCTGGCCGACGTTATTTTTAA
CGGCATGCGCCAGCAGTGCGCGCGCCTCGCGGCCGCATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10474.98 Da        Isoelectric Point: 7.8283

>AT56157 WP_007854617.1 NZ_CP012255:c90410-90129 [Cronobacter sakazakii]
MNTRYPLKTLSQLRPLLVGFRKKQGLTQKEVSARLGVTQQTYARLEANPETASIERLFRVFNVLGIEMTLCETQAAEPDA
PACDDSPAHREAW

Download         Length: 282 bp

>AT56157 NZ_CP012255:c90410-90129 [Cronobacter sakazakii]
ATGAACACCCGGTATCCACTGAAAACGCTCAGCCAGCTGCGCCCGCTGCTGGTGGGCTTTCGTAAAAAACAGGGGCTGAC
CCAGAAAGAAGTTTCGGCGCGGCTTGGCGTCACGCAGCAAACCTATGCGCGGCTGGAGGCGAACCCCGAGACGGCCAGCA
TTGAGCGCCTGTTCCGCGTCTTTAACGTGCTGGGCATTGAGATGACGCTGTGCGAAACGCAGGCGGCGGAGCCTGACGCG
CCTGCGTGTGACGATTCGCCTGCCCACCGCGAGGCGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References