Detailed information of TA system TA056156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 14677..15489
• Replicon: plasmid pCS1
• Accession: NZ_CP012254
• Organism: Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: AFK64_RS20040
• Protein ID: WP_192575777.1
• Coordinates: 14677..15195 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A853H7H9
• Locus tag: AFK64_RS20045
• Protein ID: WP_029463984.1
• Coordinates: 15220..15489 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK64_RS20015	9876..10523	+	648	WP_029590316.1	hypothetical protein	-
AFK64_RS20020	11996..13123	+	1128	Protein_8	tail fiber domain-containing protein	-
AFK64_RS20025	13213..13536	+	324	WP_029463980.1	hypothetical protein	-
AFK64_RS20030	13548..14240	+	693	WP_029590318.1	membrane protein	-
AFK64_RS20035	14242..14493	+	252	WP_032803499.1	hypothetical protein	-
AFK64_RS20040	14677..15195	-	519	WP_192575777.1	GNAT family N-acetyltransferase	Toxin
AFK64_RS20045	15220..15489	-	270	WP_029463984.1	DUF1778 domain-containing protein	Antitoxin
AFK64_RS20050	15892..16557	+	666	WP_029590320.1	AAA family ATPase	-
AFK64_RS20055	16563..16916	+	354	WP_161576472.1	hypothetical protein	-
AFK64_RS20060	17122..18285	+	1164	WP_029590324.1	hypothetical protein	-
AFK64_RS20065	18493..19218	+	726	WP_029463882.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110093	110093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19245.83 Da
• Isoelectric Point: 6.9550

>T56156 WP_192575777.1 NZ_CP012254:c15195-14677 [Cronobacter sakazakii]
MFSEEAVYDFSDFDCGEASLNEFLKNRLAQQHSGRILRGYLLLTKDAIPKVKGFYTLSGSCFARQTLPSNTQQRKIPYSD
APSVTLGRLAIDKSIQRQGKGEELVVDAMKVVYQASRAVGIYALFVDAKNPAARQFYQNLGFIPLKGENANSLFYPTKSI
EALFEEKPSEDE

Nucleotide

• Length: 519 bp

>T56156 NZ_CP012254:c15195-14677 [Cronobacter sakazakii]
ATGTTTTCAGAAGAAGCCGTATACGACTTCTCAGACTTCGACTGTGGCGAAGCATCTCTAAATGAGTTTCTTAAAAATCG
CCTGGCACAGCAACACAGCGGGCGTATATTGCGCGGTTATCTGCTTCTGACCAAAGACGCGATACCGAAAGTTAAAGGGT
TTTACACGCTGTCCGGGAGCTGTTTCGCAAGACAAACGCTCCCATCCAACACGCAGCAGCGGAAGATACCTTATTCTGAT
GCCCCCAGCGTTACGCTCGGACGTCTGGCGATAGACAAGAGCATTCAACGGCAAGGTAAAGGTGAAGAATTGGTGGTAGA
TGCCATGAAAGTGGTCTACCAGGCTTCTCGTGCCGTTGGTATCTACGCGCTGTTTGTAGATGCAAAAAATCCGGCCGCCA
GGCAGTTTTACCAGAATCTGGGGTTTATCCCTCTGAAGGGTGAAAACGCCAACTCGCTTTTCTATCCAACCAAAAGCATT
GAGGCTCTGTTTGAAGAGAAGCCGTCTGAAGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10153.64 Da
• Isoelectric Point: 7.0160

>AT56156 WP_029463984.1 NZ_CP012254:c15489-15220 [Cronobacter sakazakii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMNALSNPPAPNERLKR
AAKRLQNME

Nucleotide

• Length: 270 bp

>AT56156 NZ_CP012254:c15489-15220 [Cronobacter sakazakii]
ATGTCCGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACTATCACACAGTTCATGGTTGCCAGCGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATCCTCAGTGAAGAGTCCTGGAATCTGGTAATGAACGCTCTCAGTAACCCGCCTGCACCGAATGAAAGGCTGAAACGA
GCTGCCAAGCGTCTACAAAATATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A853H7H9