Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2803271..2803890 Replicon chromosome
Accession NZ_CP012253
Organism Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)


Gene name Hha Uniprot ID A0A2S9UIC9
Locus tag AFK64_RS13085 Protein ID WP_004386909.1
Coordinates 2803672..2803890 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q9CL77
Locus tag AFK64_RS13080 Protein ID WP_004386908.1
Coordinates 2803271..2803645 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK64_RS13070 2798432..2799637 + 1206 WP_007848022.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AFK64_RS13075 2799659..2802808 + 3150 WP_007848020.1 multidrug efflux RND transporter permease subunit -
AFK64_RS13080 2803271..2803645 + 375 WP_004386908.1 Hha toxicity modulator TomB Antitoxin
AFK64_RS13085 2803672..2803890 + 219 WP_004386909.1 hemolysin expression modulator Hha Toxin
AFK64_RS13090 2804099..2804650 + 552 WP_004386910.1 maltose O-acetyltransferase -
AFK64_RS13095 2804770..2805279 + 510 WP_007891920.1 YlaC family protein -
AFK64_RS22110 2805308..2805448 - 141 WP_004386911.1 type B 50S ribosomal protein L36 -
AFK64_RS13100 2805452..2805712 - 261 WP_004386912.1 type B 50S ribosomal protein L31 -
AFK64_RS13105 2805853..2807754 - 1902 WP_014729414.1 methyl-accepting chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8598.02 Da        Isoelectric Point: 9.4828

>T56155 WP_004386909.1 NZ_CP012253:2803672-2803890 [Cronobacter sakazakii]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Download         Length: 219 bp

>T56155 NZ_CP012253:2803672-2803890 [Cronobacter sakazakii]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGAGACGTTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTATCCGACAACGAACTGGCGGTATTTTATTCAGCGGCGGATCATCGTCTGGCTGAAC
TGACGATGAATAAGCTCTACGATAAAATTCCGACCGCCGTGTGGAAGTTCGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14343.05 Da        Isoelectric Point: 4.7168

>AT56155 WP_004386908.1 NZ_CP012253:2803271-2803645 [Cronobacter sakazakii]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFGLNYKIKHEDDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFVNASKENPVSLSF

Download         Length: 375 bp

>AT56155 NZ_CP012253:2803271-2803645 [Cronobacter sakazakii]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTTTGCGAAAGCCTGTATCATGATTGCCTTGC
CACGCTCGGTGAAAACCAGCATGGCTGGGTTAACGACCCCACTTCTGCCGTTAACCTGCAACTCAACGACTTGATTGAGC
ATATTGCCAGCTTCGGCCTGAATTATAAAATTAAGCATGAAGATGACGCAGCTCTCATTGAGCAATTAGATGAATATCTG
GATGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTCGTAAACGCCAGCAAAGAAAACCCTGTAAGCTTGTCTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S9UIC9

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q9CL77

References