Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 871245..871909 Replicon chromosome
Accession NZ_CP012253
Organism Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)


Gene name ykfI Uniprot ID A0A157QTP3
Locus tag AFK64_RS04070 Protein ID WP_007869731.1
Coordinates 871245..871571 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0M7FI99
Locus tag AFK64_RS04075 Protein ID WP_007869729.1
Coordinates 871592..871909 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK64_RS22490 866897..867127 + 231 WP_007847728.1 hypothetical protein -
AFK64_RS04055 867131..867490 + 360 WP_007847727.1 hypothetical protein -
AFK64_RS04060 867614..868309 - 696 WP_004385019.1 aquaporin Z -
AFK64_RS04065 869754..870773 - 1020 WP_029590959.1 acyltransferase -
AFK64_RS04070 871245..871571 - 327 WP_007869731.1 TA system toxin CbtA family protein Toxin
AFK64_RS04075 871592..871909 - 318 WP_007869729.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AFK64_RS04080 871923..872150 - 228 WP_013098172.1 DUF987 domain-containing protein -
AFK64_RS04085 872159..872635 - 477 WP_013098171.1 RadC family protein -
AFK64_RS04090 872651..873109 - 459 WP_013098170.1 antirestriction protein -
AFK64_RS04095 873213..873452 - 240 WP_007869725.1 DUF905 domain-containing protein -
AFK64_RS04100 873476..874297 - 822 WP_007869723.1 DUF945 domain-containing protein -
AFK64_RS04105 874424..874684 - 261 WP_007869721.1 hypothetical protein -
AFK64_RS04110 874764..875036 - 273 WP_007869719.1 hypothetical protein -
AFK64_RS04115 875093..875830 - 738 WP_013098169.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 866579..898340 31761


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-106)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12299.25 Da        Isoelectric Point: 8.9951

>T56143 WP_007869731.1 NZ_CP012253:c871571-871245 [Cronobacter sakazakii]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Download         Length: 327 bp

>T56143 NZ_CP012253:c871571-871245 [Cronobacter sakazakii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCAGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGGC
GCGTTTGCTGGAACAGCACTACGGTCTGACAATAAACGACACCCCATTCAGCGATGAAGTTGTTATACAGCAACATATCG
ATGCCGGTATCACCCTGTCAGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
CGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGAGGGCAAGACAGGCAATAGGTCTTTTACGAGGATC
TCATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11917.60 Da        Isoelectric Point: 7.0264

>AT56143 WP_007869729.1 NZ_CP012253:c871909-871592 [Cronobacter sakazakii]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT56143 NZ_CP012253:c871909-871592 [Cronobacter sakazakii]
ATGACCCACATCATCTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGGCT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGCCGGAAGCTGGATGACACATTCCCAC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A157QTP3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M7FI99

References