Detailed information of TA system TA056142

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 597927..598611
• Replicon: chromosome
• Accession: NZ_CP012253
• Organism: Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AFK64_RS02855
• Protein ID: WP_179141210.1
• Coordinates: 597927..598250 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AFK64_RS02860
• Protein ID: WP_015386412.1
• Coordinates: 598288..598611 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK64_RS02850	594412..597543	+	3132	WP_004386116.1	type I restriction endonuclease subunit R	-
AFK64_RS02855	597927..598250	-	324	WP_179141210.1	TA system toxin CbtA family protein	Toxin
AFK64_RS02860	598288..598611	-	324	WP_015386412.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AFK64_RS02865	598633..598854	-	222	WP_015386413.1	DUF987 family protein	-
AFK64_RS02870	598863..599336	-	474	WP_015386414.1	DNA repair protein RadC	-
AFK64_RS02875	599369..600187	-	819	WP_015386415.1	DUF945 domain-containing protein	-
AFK64_RS22710	600455..601771	+	1317	WP_071813833.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	592667..613989	21322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (10-106)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12152.91 Da
• Isoelectric Point: 6.7235

>T56142 WP_179141210.1 NZ_CP012253:c598250-597927 [Cronobacter sakazakii]
ISSVPATVPVSSRLSPVKVWQQLLTYLLEQHYGLTLNDTLFHDDTAIQEHIDAGITLADAVNFLVEKYELVRIDRKGFSW
QEQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 324 bp

>T56142 NZ_CP012253:c598250-597927 [Cronobacter sakazakii]
ATTTCATCTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCGCCCGTGAAGGTCTGGCAGCAACTGTTGACGTATCT
GCTGGAGCAGCACTACGGCCTGACTCTCAACGACACACTATTTCATGACGACACGGCCATTCAGGAGCATATCGATGCGG
GAATAACGCTTGCCGATGCTGTTAATTTCCTGGTCGAAAAGTACGAACTGGTTCGTATCGATCGGAAGGGATTTTCATGG
CAAGAACAAACCCCGTATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAA
ATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11953.66 Da
• Isoelectric Point: 7.0268

>AT56142 WP_015386412.1 NZ_CP012253:c598611-598288 [Cronobacter sakazakii]
MQNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGTFNKDEALRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLHHN
GLTCEADTLGSHGYVYIVIYPQASATQ

Nucleotide

• Length: 324 bp

>AT56142 NZ_CP012253:c598611-598288 [Cronobacter sakazakii]
ATGCAAAATTCATGGGGACTGAAGCGCGACATCTCGCCGTGCTTCGGTGCAAGACTTGTGCAGGAAGGCTGTCGCCTGCA
TTTTCTGGCGGACCGTGCGGGTATTAGCGGTACGTTTAACAAGGACGAGGCGCTGCGTCTGGATCAGGCGTTTCCGCTAG
TGATGAAACAGCTTGAATTGATGCTGACAACCGGTGAACTGAATCCGCGCCATCAGCATTGCGTTACGTTGCATCATAAC
GGACTCACCTGCGAGGCTGATACTCTGGGCTCTCACGGATATGTTTACATCGTTATTTATCCGCAGGCATCTGCAACCCA
GTAA